LOCUS       BBa_I13554              1944 bp    DNA     linear       25-Nov-2024
DEFINITION  AHL-induced GFP
ACCESSION   BBa_I13554
VERSION     BBa_I13554.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..1807
                     /label=GFP protein
     CDS             1088..1807
                     /label=BBa_E0040
     stem_loop       1827..1870
                     /label=stem_loop
     misc_feature    1816..1895
                     /label=BBa_B0010
     terminator      1816..1895
                     /label=BBa_B0010
     stem_loop       1911..1930
                     /label=T7 TE
     misc_feature    1937..1937
                     /label=stop
     polyA_site      1931..1944
                     /label=polya
     misc_feature    1904..1944
                     /label=BBa_B0012
     terminator      1904..1944
                     /label=BBa_B0012
     misc_feature    1070..1944
                     /label=BBa_I13504
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     1141 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     1201 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1261 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
     1321 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     1381 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     1441 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     1501 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     1561 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1621 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1681 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     1741 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1801 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1861 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1921 cgggtgggcc tttctgcgtt tata
//