LOCUS       BBa_I13558              1788 bp    DNA     linear       12-May-2024
DEFINITION  Ptet-driven expression of GFP and RFP
ACCESSION   BBa_I13558
VERSION     BBa_I13558.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     misc_feature    63..800
                     /label=BBa_I13500
     misc_feature    809..829
                     /label=RNAse E site
     misc_feature    817..818
                     /label=RNAse E cut site (between the bases)
     misc_feature    830..863
                     /label=Insertion site
     misc_feature    864..884
                     /label=RNAse E site
     misc_feature    872..873
                     /label=RNAse E cut site (between the bases)
     stem_loop       885..919
                     /label=Hairpin
     misc_feature    809..919
                     /label=BBa_I13457
     misc_feature    932..935
                     /label=conserved
     RBS             928..939
                     /label=BBa_B0034
     CDS             946..1620
                     /label=mrfp1
     misc_feature    1627..1651
                     /label=Help:Barcodes
     CDS             946..1626
                     /label=BBa_E1010
     stem_loop       1671..1714
                     /label=stem_loop
     misc_feature    1660..1739
                     /label=BBa_B0010
     terminator      1660..1739
                     /label=BBa_B0010
     stem_loop       1755..1774
                     /label=T7 TE
     misc_feature    1781..1781
                     /label=stop
     polyA_site      1775..1788
                     /label=polya
     misc_feature    1748..1788
                     /label=BBa_B0012
     terminator      1748..1788
                     /label=BBa_B0012
     misc_feature    928..1788
                     /label=BBa_I13507
     misc_feature    809..1788
                     /label=BBa_I13528
     misc_feature    63..1788
                     /label=BBa_I13545
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagta ttatttgtat tgatctggtg aattaggtct
      841 tcgaatccga tccattgcag tggtattatt tgtattgatc tccttactat ctctcgagag
      901 attagtacct ttggagatct actagagaaa gaggagaaat actagatggc ttcctccgaa
      961 gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac
     1021 gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa
     1081 ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc
     1141 cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg
     1201 tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt
     1261 accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt
     1321 ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct
     1381 tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg
     1441 aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa
     1501 ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac
     1561 gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct
     1621 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     1681 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1741 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//