##gff-version 3
##sequence-region BBa_I13558 1 1788
BBa_I13558	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I13558	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13558	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13558	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13558	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13558	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13558	.	engineered_region	63	1788	.	+	0	ID=BBa_I13545;Name=BBa_I13545
BBa_I13558	.	engineered_region	809	1788	.	+	0	ID=BBa_I13528;Name=BBa_I13528;Parent=BBa_I13545
BBa_I13558	.	engineered_region	928	1788	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_I13528
BBa_I13558	.	CDS	946	1626	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_I13558	.	engineered_tag	1627	1651	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13558	.	CDS	946	1620	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13558	.	terminator	1748	1788	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_I13558	.	polyA_site	1775	1788	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13558	.	engineered_region	1748	1788	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13558	.	stop_codon	1781	1781	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13558	.	stem_loop	1755	1774	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13558	.	ribosome_entry_site	928	939	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_I13558	.	conserved_region	932	935	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13558	.	terminator	1660	1739	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_I13558	.	engineered_region	1660	1739	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13558	.	stem_loop	1671	1714	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13558	.	sequence_feature	809	919	.	+	0	ID=BBa_I13457;Name=BBa_I13457;Parent=BBa_I13528
BBa_I13558	.	sequence_feature	817	818	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13558	.	stem_loop	885	919	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13558	.	sequence_feature	830	863	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
BBa_I13558	.	sequence_feature	809	829	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
BBa_I13558	.	sequence_feature	872	873	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13558	.	sequence_feature	864	884	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
BBa_I13558	.	engineered_region	63	800	.	+	0	ID=BBa_I13500;Name=BBa_I13500;Parent=BBa_I13545
BBa_I13558	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13500
BBa_I13558	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13558	.	CDS	81	800	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13500
BBa_I13558	.	CDS	81	800	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_I13558
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaataataatactagagtattatttgtattgatctggtgaattaggtcttcgaatccgatccattgcagtggtattat
ttgtattgatctccttactatctctcgagagattagtacctttggagatctactagagaaagaggagaaatactagatg
gcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcg
aaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgct
gccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccg
gactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccg
ttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacgg
tccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggt
gaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaac
cggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttga
acagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata