LOCUS       BBa_I13561              1607 bp    DNA     linear       22-Dec-2024
DEFINITION  polycistronic mRFP1 and GFP
ACCESSION   BBa_I13561
VERSION     BBa_I13561.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     misc_feature    1..738
                     /label=BBa_I13500
     misc_feature    751..754
                     /label=conserved
     RBS             747..758
                     /label=BBa_B0034
     CDS             765..1439
                     /label=mrfp1
     misc_feature    1446..1470
                     /label=Help:Barcodes
     CDS             765..1445
                     /label=BBa_E1010
     stem_loop       1490..1533
                     /label=stem_loop
     misc_feature    1479..1558
                     /label=BBa_B0010
     terminator      1479..1558
                     /label=BBa_B0010
     stem_loop       1574..1593
                     /label=T7 TE
     misc_feature    1600..1600
                     /label=stop
     polyA_site      1594..1607
                     /label=polya
     misc_feature    1567..1607
                     /label=BBa_B0012
     terminator      1567..1607
                     /label=BBa_B0012
     misc_feature    747..1607
                     /label=BBa_I13507
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagaaag aggagaaata ctagatggct tcctccgaag
      781 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
      841 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
      901 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
      961 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
     1021 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
     1081 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
     1141 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
     1201 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
     1261 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
     1321 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
     1381 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
     1441 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
     1501 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1561 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//