LOCUS       BBa_I13562              1615 bp    DNA     linear       26-Dec-2024
DEFINITION  polycistronic mCherry and GFP
ACCESSION   BBa_I13562
VERSION     BBa_I13562.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..729
                     /label=mCherry
     misc_feature    370..370
                     /label=C->T (removing PstI site)
     misc_feature    19..732
                     /label=BBa_J06504
     misc_feature    745..748
                     /label=conserved
     RBS             741..752
                     /label=BBa_B0034
     CDS             759..1478
                     /label=GFP protein
     CDS             759..1478
                     /label=BBa_E0040
     stem_loop       1498..1541
                     /label=stem_loop
     misc_feature    1487..1566
                     /label=BBa_B0010
     terminator      1487..1566
                     /label=BBa_B0010
     stem_loop       1582..1601
                     /label=T7 TE
     misc_feature    1608..1608
                     /label=stop
     polyA_site      1602..1615
                     /label=polya
     misc_feature    1575..1615
                     /label=BBa_B0012
     terminator      1575..1615
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaagtaat aatactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt
      781 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      841 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      901 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
      961 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
     1021 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     1081 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     1141 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     1201 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     1261 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     1321 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     1381 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     1441 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
     1501 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1561 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//