LOCUS BBa_I13564 2838 bp DNA linear 23-Dec-2024 DEFINITION Screening Plasmid 1.1 ACCESSION BBa_I13564 VERSION BBa_I13564.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..1807 /label=GFP protein CDS 1088..1807 /label=BBa_E0040 misc_feature 1816..1836 /label=RNaseE site misc_feature 1837..1854 /label=SPACER misc_feature 1855..1876 /label=prefix misc_feature 1877..1897 /label=suffix misc_feature 1898..1913 /label=SPACER misc_feature 1914..1934 /label=RNaseE site stem_loop 1935..1969 /label=hairpin misc_feature 1816..1969 /label=BBa_I13563 misc_feature 1982..1985 /label=conserved RBS 1978..1989 /label=BBa_B0034 CDS 1996..2670 /label=mrfp1 misc_feature 2677..2701 /label=Help:Barcodes CDS 1996..2701 /label=BBa_E1010 stem_loop 2721..2764 /label=stem_loop misc_feature 2710..2789 /label=BBa_B0010 terminator 2710..2789 /label=BBa_B0010 stem_loop 2805..2824 /label=T7 TE misc_feature 2831..2831 /label=stop polyA_site 2825..2838 /label=polya misc_feature 2798..2838 /label=BBa_B0012 terminator 2798..2838 /label=BBa_B0012 terminator 2710..2838 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt 1141 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac 1201 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc 1261 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat 1321 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat 1381 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac 1441 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg 1501 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa 1561 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact 1621 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 1681 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat 1741 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa 1801 ataataatac tagagtatta tttgtattga tctggtgaat taggtcttcg aatcgaattc 1861 gcggccgctt ctagagtact agtagcggcc gctgcagcga tccattgcag tggtattatt 1921 tgtattgatc tccttactat ctctcgagag attagtacct ttggagatct actagagaaa 1981 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa 2041 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt 2101 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg 2161 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac 2221 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt 2281 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac 2341 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt 2401 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt 2461 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct 2521 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc 2581 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa 2641 cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg 2701 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2761 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2821 ggcctttctg cgtttata //