##gff-version 3
##sequence-region BBa_I13573 1 966
BBa_I13573	.	terminator	838	966	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I13573	.	terminator	838	917	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13573	.	stem_loop	849	892	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13573	.	engineered_region	838	917	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13573	.	terminator	926	966	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13573	.	polyA_site	953	966	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13573	.	stem_loop	933	952	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13573	.	engineered_region	926	966	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13573	.	stop_codon	959	959	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13573	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I13573	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13573	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13573	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13573	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13573	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13573	.	CDS	124	829	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_I13573	.	CDS	124	798	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13573	.	engineered_tag	805	829	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13573	.	mature_transcript_region	63	97	.	+	0	ID=BBa_I13577;Name=BBa_I13577
BBa_I13573	.	stem_loop	63	97	.	+	0	ID=annotation1934262;Name=Hairpin;Parent=BBa_I13577
BBa_I13573	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13573	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13573
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtactatctctcgagaga
ttagtacctttggagatctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcat
gcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtac
gaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagt
tccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggttt
caaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgag
ttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggg
aagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacgg
tggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgac
atcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactcca
ccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata