LOCUS       BBa_I13581              2264 bp    DNA     linear       25-Nov-2024
DEFINITION  AHL-inducible promoter.S.ccdB.X.GFP
ACCESSION   BBa_I13581
VERSION     BBa_I13581.1  
FEATURES             Location/Qualifiers
     misc_feature    1..8
                     /label=BBa_G0007
     misc_feature    9..14
                     /label=MfeI site
     misc_feature    9..14
                     /label=BBa_B0047
     misc_feature    16..34
                     /label=TetR 1
     misc_feature    16..69
                     /label=BBa_R0040
     promoter        35..40
                     /label=-35
     misc_feature    41..59
                     /label=TetR 2
     promoter        58..63
                     /label=-10
     promoter        16..69
                     /label=BBa_R0040
     misc_feature    82..85
                     /label=conserved
     RBS             78..89
                     /label=BBa_B0034
     misc_feature    96..97
                     /label=prefix
     misc_feature    269..269
                     /label=T
     misc_feature    587..587
                     /label=A
     CDS             96..845
                     /label=luxR
     misc_feature    96..851
                     /label=BBa_C0062
     misc_feature    852..876
                     /label=Help:Barcodes
     CDS             96..851
                     /label=BBa_C0062
     stem_loop       896..939
                     /label=stem_loop
     misc_feature    885..964
                     /label=BBa_B0010
     terminator      885..964
                     /label=BBa_B0010
     stem_loop       980..999
                     /label=T7 TE
     misc_feature    1006..1006
                     /label=stop
     polyA_site      1000..1013
                     /label=polya
     misc_feature    973..1013
                     /label=BBa_B0012
     terminator      973..1013
                     /label=BBa_B0012
     terminator      885..1013
                     /label=BBa_B0015
     misc_feature    1022..1041
                     /label=LuxR/HSL
     promoter        1041..1046
                     /label=-35
     promoter        1063..1068
                     /label=-10
     misc_feature    1074..1074
                     /label=start
     misc_feature    1022..1076
                     /label=BBa_R0062
     promoter        1022..1076
                     /label=BBa_R0062
     misc_feature    16..1076
                     /label=BBa_F2620
     misc_feature    1078..1083
                     /label=BBa_M31992
     promoter        1125..1130
                     /label=-35
     promoter        1148..1153
                     /label=-10
     CDS             1192..1500
                     /label=ccdB
     misc_feature    1085..1500
                     /label=BBa_P1016
     misc_feature    1502..1507
                     /label=BBa_M31991
     RBS             1509..1523
                     /label=RBS-1\Strong
     RBS             1516..1520
                     /label=RBS
     misc_feature    1509..1523
                     /label=BBa_B0030
     RBS             1509..1523
                     /label=BBa_B0030
     misc_feature    1524..1529
                     /label=BBa_G0000
     CDS             1530..2249
                     /label=GFP protein
     CDS             1530..2249
                     /label=BBa_E0040
     misc_feature    2251..2256
                     /label=NsiI site
     misc_feature    2251..2256
                     /label=BBa_B0046
     misc_feature    2257..2264
                     /label=BBa_G0008
ORIGIN
        1 gtttcttcca attggtccct atcagtgata gagattgaca tccctatcag tgatagagat
       61 actgagcact actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca
      121 catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct
      181 tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc
      241 attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat
      301 attatgatga cgctaattta ataaaatatg atcctatagt agattattct aactccaatc
      361 attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg
      421 taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg
      481 ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata
      541 gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt
      601 atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag
      661 aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt
      721 gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa
      781 accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta
      841 aaaattaata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa
      901 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      961 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     1021 gacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaatact
     1081 agtaggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     1141 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgcagttt
     1201 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     1261 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     1321 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     1381 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     1441 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataataa
     1501 ctctagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag
     1561 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1621 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1681 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     1741 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     1801 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     1861 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1921 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1981 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     2041 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     2101 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     2161 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     2221 atggcatgga tgaactatac aaataataat atgcatgaag aaac
//