BBa_I13581
type: ComponentDefinition
persistent identity: BBa I13581
display id: BBa_I13581
version: 1
was generated by: iGEM to SBOL conversion
description: AHL-inducible promoter.S.ccdB.X.GFP
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:35Z
mutable provenance: This is a composite part of BBa_F2620, BBa_P1016, BBa_E0840. However, the DNA sequence of the protein coding regions may be changed to facilitate the DNA fabrication process.
owned by: james
owned by: myers
mutable description: This is a measurement plasmid consisting of an AHL-inducible promoter (BBa_F2620), a SpeI site, ccdB, an XbaI site, and a GFP reporter (BBa_E0840). It allows cloning of any device whose input and output are PoPS. The assembled constructs allows tunable control of the input PoPS signal (via AHL) and measurement of the output PoPS signal (via fluorescence).
discontinued: true
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: This measurement plasmid facilitates PoPS->PoPS device characterization. Cloning of a device using XbaI and SpeI into this plasmid allows construction of a composite part that looks identical to a composite part that has been constructed via standard assembly.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_F2620
component: BBa_B0047
component: BBa_G0007
component: BBa_B0030
component: BBa_M31991
component: BBa_P1016
component: BBa_M31992
component: BBa_G0008
component: BBa_B0046
component: BBa_E0040
component: BBa_G0000
sequence annotation: BBa_G0007
sequence annotation: BBa_B0047
sequence annotation: BBa_F2620
sequence annotation: BBa_M31992
sequence annotation: BBa_P1016
sequence annotation: BBa_M31991
sequence annotation: BBa_B0030
sequence annotation: BBa_G0000
sequence annotation: BBa_E0040
sequence annotation: BBa_B0046
sequence annotation: BBa_G0008
sequence: https://synbiohub.org/public/igem/BBa_I13581_sequence/1

BBa_F2620
type: Component
persistent identity: component2322274
display id: component2322274
version: 1
top level: BBa_I13581
definition: BBa_F2620
access: public

BBa_B0047
type: Component
persistent identity: component2322248
display id: component2322248
version: 1
top level: BBa_I13581
definition: MfeI
access: public

BBa_G0007
type: Component
persistent identity: component2322246
display id: component2322246
version: 1
top level: BBa_I13581
definition: BBa_G0007
access: public

BBa_B0030
type: Component
persistent identity: component2322282
display id: component2322282
version: 1
top level: BBa_I13581
definition: BBa_B0030
access: public

BBa_M31991
type: Component
persistent identity: component2322280
display id: component2322280
version: 1
top level: BBa_I13581
definition: XbaI
access: public

BBa_P1016
type: Component
persistent identity: component2322279
display id: component2322279
version: 1
top level: BBa_I13581
definition: ccdB
access: public

BBa_M31992
type: Component
persistent identity: component2322275
display id: component2322275
version: 1
top level: BBa_I13581
definition: SpeI
access: public

BBa_G0008
type: Component
persistent identity: component2322289
display id: component2322289
version: 1
top level: BBa_I13581
definition: BBa_G0008
access: public

BBa_B0046
type: Component
persistent identity: component2322288
display id: component2322288
version: 1
top level: BBa_I13581
definition: NsiI
access: public

BBa_E0040
type: Component
persistent identity: component2322286
display id: component2322286
version: 1
top level: BBa_I13581
definition: GFP
access: public

BBa_G0000
type: Component
persistent identity: component2322284
display id: component2322284
version: 1
top level: BBa_I13581
definition: scar
access: public

BBa_G0007
type: SequenceAnnotation
persistent identity: annotation2322246
display id: annotation2322246
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322246/range2322246/1
component: BBa_G0007

https://synbiohub.org/public/igem/BBa_I13581/annotation2322246/range2322246/1
type: Range
persistent identity: range2322246
display id: range2322246
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 1
end: 8
orientation: inline

BBa_B0047
type: SequenceAnnotation
persistent identity: annotation2322248
display id: annotation2322248
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322248/range2322248/1
component: BBa_B0047

https://synbiohub.org/public/igem/BBa_I13581/annotation2322248/range2322248/1
type: Range
persistent identity: range2322248
display id: range2322248
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 9
end: 14
orientation: inline

BBa_F2620
type: SequenceAnnotation
persistent identity: annotation2322274
display id: annotation2322274
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322274/range2322274/1
component: BBa_F2620

https://synbiohub.org/public/igem/BBa_I13581/annotation2322274/range2322274/1
type: Range
persistent identity: range2322274
display id: range2322274
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 16
end: 1076
orientation: inline

BBa_M31992
type: SequenceAnnotation
persistent identity: annotation2322275
display id: annotation2322275
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322275/range2322275/1
component: BBa_M31992

https://synbiohub.org/public/igem/BBa_I13581/annotation2322275/range2322275/1
type: Range
persistent identity: range2322275
display id: range2322275
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 1078
end: 1083
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation2322279
display id: annotation2322279
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322279/range2322279/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I13581/annotation2322279/range2322279/1
type: Range
persistent identity: range2322279
display id: range2322279
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 1085
end: 1500
orientation: inline

BBa_M31991
type: SequenceAnnotation
persistent identity: annotation2322280
display id: annotation2322280
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322280/range2322280/1
component: BBa_M31991

https://synbiohub.org/public/igem/BBa_I13581/annotation2322280/range2322280/1
type: Range
persistent identity: range2322280
display id: range2322280
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 1502
end: 1507
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2322282
display id: annotation2322282
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322282/range2322282/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I13581/annotation2322282/range2322282/1
type: Range
persistent identity: range2322282
display id: range2322282
version: 1
top level: BBa_I13581
direction: direction/forward
start: 1509
end: 1523
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2322284
display id: annotation2322284
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322284/range2322284/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_I13581/annotation2322284/range2322284/1
type: Range
persistent identity: range2322284
display id: range2322284
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 1524
end: 1529
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2322286
display id: annotation2322286
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322286/range2322286/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13581/annotation2322286/range2322286/1
type: Range
persistent identity: range2322286
display id: range2322286
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 1530
end: 2249
orientation: inline

BBa_B0046
type: SequenceAnnotation
persistent identity: annotation2322288
display id: annotation2322288
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322288/range2322288/1
component: BBa_B0046

https://synbiohub.org/public/igem/BBa_I13581/annotation2322288/range2322288/1
type: Range
persistent identity: range2322288
display id: range2322288
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 2251
end: 2256
orientation: inline

BBa_G0008
type: SequenceAnnotation
persistent identity: annotation2322289
display id: annotation2322289
version: 1
top level: BBa_I13581
location: https://synbiohub.org/public/igem/BBa_I13581/annotation2322289/range2322289/1
component: BBa_G0008

https://synbiohub.org/public/igem/BBa_I13581/annotation2322289/range2322289/1
type: Range
persistent identity: range2322289
display id: range2322289
version: 1
top level: BBa_I13581
direction: direction/reverse
start: 2257
end: 2264
orientation: inline

XbaI
type: ComponentDefinition
persistent identity: BBa M31991
display id: BBa_M31991
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: XbaI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_M31991_sequence/1

BBa_G0007
type: ComponentDefinition
persistent identity: BBa G0007
display id: BBa_G0007
version: 1
was generated by: iGEM to SBOL conversion
description: Short sequence to promoter addition of 3' A overhang during PCR
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: <biblio> #Brownstein-Biotechniques-1996 pmid=8780871 </biblio>
owned by: james
owned by: myers
mutable description: This is a short sequence to promoter addition of 3' A overhang during PCR. It should be added to the 5' end of any primers in which the PCR product is going to be TA cloned. It is also a helpful spacer sequence when generating linear DNA fragments to ensure that restriction enzymes will still be able to cut.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_G0007_sequence/1

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_F2620
type: ComponentDefinition
persistent identity: BBa F2620
display id: BBa_F2620
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC6HSL -> PoPS Receiver
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: Formerly I13270
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.
discontinued: false
dominant: true
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Example Data Sheets for F2620
star: true
creator: Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_R0040
component: BBa_R0062
component: BBa_B0015
component: BBa_C0062
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0015
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_F2620_sequence/1

BBa_R0040
type: Component
persistent identity: component2259889
display id: component2259889
version: 1
top level: BBa_F2620
definition: p(tetR)
access: public

BBa_R0062
type: Component
persistent identity: component2259910
display id: component2259910
version: 1
top level: BBa_F2620
definition: lux pR
access: public

BBa_B0015
type: Component
persistent identity: component2259908
display id: component2259908
version: 1
top level: BBa_F2620
definition: BBa_B0015
access: public

BBa_C0062
type: Component
persistent identity: component2259898
display id: component2259898
version: 1
top level: BBa_F2620
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component2259895
display id: component2259895
version: 1
top level: BBa_F2620
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2259889
display id: annotation2259889
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
type: Range
persistent identity: range2259889
display id: range2259889
version: 1
top level: BBa_F2620
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2259895
display id: annotation2259895
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
type: Range
persistent identity: range2259895
display id: range2259895
version: 1
top level: BBa_F2620
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2259898
display id: annotation2259898
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
type: Range
persistent identity: range2259898
display id: range2259898
version: 1
top level: BBa_F2620
direction: direction/forward
start: 81
end: 836
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259908
display id: annotation2259908
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
type: Range
persistent identity: range2259908
display id: range2259908
version: 1
top level: BBa_F2620
direction: direction/reverse
start: 870
end: 998
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2259910
display id: annotation2259910
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
type: Range
persistent identity: range2259910
display id: range2259910
version: 1
top level: BBa_F2620
direction: direction/forward
start: 1007
end: 1061
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1016
display id: BBa_P1016
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cassette without ccdA-
created: 2007-01-29T12:00:00Z
modified: 2015-06-15T01:13:47Z
mutable provenance: This part is derived from BBa_P1010 but it lacks ccdA-.
owned by: james
owned by: myers
mutable description: This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 4206
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: -35
sequence annotation: -10
sequence annotation: ccdB
sequence: https://synbiohub.org/public/igem/BBa_P1016_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1910684
display id: annotation1910684
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
type: Range
persistent identity: range1910684
display id: range1910684
version: 1
top level: ccdB
direction: direction/forward
start: 41
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1910685
display id: annotation1910685
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
type: Range
persistent identity: range1910685
display id: range1910685
version: 1
top level: ccdB
direction: direction/forward
start: 64
end: 69
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1910686
display id: annotation1910686
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
type: Range
persistent identity: range1910686
display id: range1910686
version: 1
top level: ccdB
direction: direction/forward
start: 108
end: 416
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0000
display id: BBa_G0000
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI scar for RBS-CDS junctions
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: SpeI/XbaI scar
owned by: james
owned by: myers
mutable description: This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a shorter scar to ensure proper spacing between the RBS and CDS.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0000_sequence/1

SpeI
type: ComponentDefinition
persistent identity: BBa M31992
display id: BBa_M31992
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: SpeI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31992_sequence/1

MfeI
type: ComponentDefinition
persistent identity: BBa B0047
display id: BBa_B0047
version: 1
was generated by: iGEM to SBOL conversion
description: MfeI restriction enzyme site (EcoRI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: MfeI recognition site. Digestion with MfeI yields a compatible cohesive end to EcoRI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: MfeI site
sequence: https://synbiohub.org/public/igem/BBa_B0047_sequence/1

MfeI site
type: SequenceAnnotation
persistent identity: annotation1822740
display id: annotation1822740
version: 1
top level: MfeI
location: https://synbiohub.org/public/igem/BBa_B0047/annotation1822740/range1822740/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0047/annotation1822740/range1822740/1
type: Range
persistent identity: range1822740
display id: range1822740
version: 1
top level: MfeI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_G0008
type: ComponentDefinition
persistent identity: BBa G0008
display id: BBa_G0008
version: 1
was generated by: iGEM to SBOL conversion
description: Short sequence to promoter addition of 3' A overhang during PCR
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: <biblio> #Brownstein-Biotechniques-1996 pmid=8780871 </biblio>
owned by: james
owned by: myers
mutable description: This is a short sequence to promoter addition of 3' A overhang during PCR. It should be added to the 5' end of any primers in which the PCR product is going to be TA cloned. It is also a helpful spacer sequence when generating linear DNA fragments to ensure that restriction enzymes will still be able to cut.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is the reverse complement of BBa_G0007.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_G0008_sequence/1

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

NsiI
type: ComponentDefinition
persistent identity: BBa B0046
display id: BBa_B0046
version: 1
was generated by: iGEM to SBOL conversion
description: NsiI restriction enzyme site (PstI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NsiI recognition site. Digestion with NsiI generates a compatible cohesive end to PstI.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: NsiI site
sequence: https://synbiohub.org/public/igem/BBa_B0046_sequence/1

NsiI site
type: SequenceAnnotation
persistent identity: annotation1822730
display id: annotation1822730
version: 1
top level: NsiI
location: https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1
type: Range
persistent identity: range1822730
display id: range1822730
version: 1
top level: NsiI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_I13581_sequence/1
type: Sequence
persistent identity: BBa I13581 sequence
display id: BBa_I13581_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttcttccaattggtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagtaggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataactctagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31991_sequence/1
type: Sequence
persistent identity: BBa M31991 sequence
display id: BBa_M31991_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0007_sequence/1
type: Sequence
persistent identity: BBa G0007 sequence
display id: BBa_G0007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttcttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0008_sequence/1
type: Sequence
persistent identity: BBa G0008 sequence
display id: BBa_G0008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_F2620_sequence/1
type: Sequence
persistent identity: BBa F2620 sequence
display id: BBa_F2620_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1016_sequence/1
type: Sequence
persistent identity: BBa P1016 sequence
display id: BBa_P1016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0000_sequence/1
type: Sequence
persistent identity: BBa G0000 sequence
display id: BBa_G0000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31992_sequence/1
type: Sequence
persistent identity: BBa M31992 sequence
display id: BBa_M31992_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0047_sequence/1
type: Sequence
persistent identity: BBa B0047 sequence
display id: BBa_B0047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0046_sequence/1
type: Sequence
persistent identity: BBa B0046 sequence
display id: BBa_B0046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z