LOCUS       BBa_I13607              1967 bp    DNA     linear       14-May-2024
DEFINITION  Lac YFP Tet CFP (Ly+Tc+)
ACCESSION   BBa_I13607
VERSION     BBa_I13607.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..843
                     /label=YFP (LVA)
     misc_feature    838..843
                     /label=2
     misc_feature    799..837
                     /label=SsrA
     misc_feature    323..323
                     /label=A (Q->L)
     misc_feature    82..843
                     /label=BBa_E0032
     misc_feature    82..843
                     /label=BBa_E0032
     stem_loop       863..906
                     /label=stem_loop
     misc_feature    852..931
                     /label=BBa_B0010
     terminator      852..931
                     /label=BBa_B0010
     stem_loop       947..966
                     /label=T7 TE
     misc_feature    973..973
                     /label=stop
     polyA_site      967..980
                     /label=polya
     misc_feature    940..980
                     /label=BBa_B0012
     terminator      940..980
                     /label=BBa_B0012
     misc_feature    64..980
                     /label=BBa_E0432
     misc_feature    1..980
                     /label=BBa_I6061
     misc_feature    989..1007
                     /label=TetR 1
     misc_feature    989..1042
                     /label=BBa_R0040
     promoter        1008..1013
                     /label=-35
     misc_feature    1014..1032
                     /label=TetR 2
     promoter        1031..1036
                     /label=-10
     promoter        989..1042
                     /label=BBa_R0040
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1069..1830
                     /label=CFP (LVA)
     misc_feature    1787..1824
                     /label=SsrA
     misc_feature    1825..1830
                     /label=2
     misc_feature    1137..1137
                     /label=A
     misc_feature    1069..1830
                     /label=BBa_E0022
     misc_feature    1069..1830
                     /label=BBa_E0022
     stem_loop       1850..1893
                     /label=stem_loop
     misc_feature    1839..1918
                     /label=BBa_B0010
     terminator      1839..1918
                     /label=BBa_B0010
     stem_loop       1934..1953
                     /label=T7 TE
     misc_feature    1960..1960
                     /label=stop
     polyA_site      1954..1967
                     /label=polya
     misc_feature    1927..1967
                     /label=BBa_B0012
     terminator      1927..1967
                     /label=BBa_B0012
     misc_feature    1051..1967
                     /label=BBa_E0422
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
      301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa
      841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      961 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat
     1021 cagtgataga gatactgagc actactagag aaagaggaga aatactagat ggtgagcaag
     1081 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac
     1141 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1201 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1261 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1321 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1381 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1441 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1501 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1561 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1621 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1681 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1741 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac
     1801 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa
     1861 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1921 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//