LOCUS       BBa_I13613              2282 bp    DNA     linear       17-May-2024
DEFINITION  Cascaded Tet (medium) and Lac (weak) QPIs
ACCESSION   BBa_I13613
VERSION     BBa_I13613.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             24..640
                     /label=tetR
     misc_feature    641..674
                     /label=SsrA
     misc_feature    681..705
                     /label=Help:Barcodes
     CDS             21..680
                     /label=BBa_C0040
     stem_loop       725..768
                     /label=stem_loop
     misc_feature    714..793
                     /label=BBa_B0010
     terminator      714..793
                     /label=BBa_B0010
     stem_loop       809..828
                     /label=T7 TE
     misc_feature    835..835
                     /label=stop
     polyA_site      829..842
                     /label=polya
     misc_feature    802..842
                     /label=BBa_B0012
     terminator      802..842
                     /label=BBa_B0012
     misc_feature    851..869
                     /label=TetR 1
     misc_feature    851..904
                     /label=BBa_R0040
     promoter        870..875
                     /label=-35
     misc_feature    876..894
                     /label=TetR 2
     promoter        893..898
                     /label=-10
     promoter        851..904
                     /label=BBa_R0040
     misc_feature    1..904
                     /label=BBa_Q01400
     RBS             913..923
                     /label=RBS-4\Weaker
     RBS             919..922
                     /label=RBS
     misc_feature    913..923
                     /label=BBa_B0033
     RBS             913..923
                     /label=BBa_B0033
     misc_feature    2019..2057
                     /label=LVA
     CDS             930..2057
                     /label=lacI-LVA
     misc_feature    930..2057
                     /label=BBa_C0012
     misc_feature    2058..2082
                     /label=Help:Barcodes
     CDS             930..2057
                     /label=BBa_C0012
     stem_loop       2102..2145
                     /label=stem_loop
     misc_feature    2091..2170
                     /label=BBa_B0010
     terminator      2091..2170
                     /label=BBa_B0010
     stem_loop       2186..2205
                     /label=T7 TE
     misc_feature    2212..2212
                     /label=stop
     polyA_site      2206..2219
                     /label=polya
     misc_feature    2179..2219
                     /label=BBa_B0012
     terminator      2179..2219
                     /label=BBa_B0012
     terminator      2091..2219
                     /label=BBa_B0015
     misc_feature    2230..2246
                     /label=lac O1
     promoter        2247..2252
                     /label=-35
     misc_feature    2253..2269
                     /label=lac O1
     promoter        2270..2275
                     /label=-10
     misc_feature    2228..2281
                     /label=BBa_R0011
     promoter        2228..2281
                     /label=BBa_R0011
     misc_feature    913..2282
                     /label=BBa_Q03121
ORIGIN
        1 tcacacagga aacctactag atgtccagat tagataaaag taaagtgatt aacagcgcat
       61 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      121 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      181 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      241 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      301 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      361 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      421 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      481 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      541 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      601 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      661 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca
      721 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      781 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      841 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      901 gcactactag agtcacacag gactactaga tggtgaatgt gaaaccagta acgttatacg
      961 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     1021 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     1081 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     1141 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     1201 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     1261 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     1321 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     1381 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     1441 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     1501 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     1561 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     1621 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     1681 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     1741 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     1801 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     1861 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     1921 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1981 agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg
     2041 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca
     2101 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2161 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     2221 actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag atactgagca
     2281 ca
//