##gff-version 3
##sequence-region BBa_I13621 1 2282
BBa_I13621	.	engineered_region	1	1370	.	+	0	ID=BBa_Q03121;Name=BBa_Q03121
BBa_I13621	.	CDS	18	1145	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q03121
BBa_I13621	.	engineered_tag	1146	1170	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13621	.	CDS	18	1145	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13621	.	tag	1107	1145	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13621	.	engineered_region	18	1145	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13621	.	ribosome_entry_site	1	11	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_Q03121
BBa_I13621	.	engineered_region	1	11	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_I13621	.	ribosome_entry_site	1	11	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_I13621	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_I13621	.	promoter	1316	1369	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q03121
BBa_I13621	.	promoter	1358	1363	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13621	.	engineered_region	1316	1369	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13621	.	promoter	1335	1340	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13621	.	operator	1318	1334	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13621	.	operator	1341	1357	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13621	.	terminator	1179	1307	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q03121
BBa_I13621	.	terminator	1267	1307	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13621	.	engineered_region	1267	1307	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13621	.	stop_codon	1300	1300	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13621	.	polyA_site	1294	1307	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13621	.	stem_loop	1274	1293	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13621	.	terminator	1179	1258	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13621	.	engineered_region	1179	1258	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13621	.	stem_loop	1190	1233	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13621	.	engineered_region	1379	2282	.	+	0	ID=BBa_Q01400;Name=BBa_Q01400
BBa_I13621	.	ribosome_entry_site	1379	1392	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01400
BBa_I13621	.	conserved_region	1385	1388	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13621	.	CDS	1399	2058	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q01400
BBa_I13621	.	tag	2019	2052	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13621	.	engineered_tag	2059	2083	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13621	.	CDS	1402	2018	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13621	.	terminator	2180	2220	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q01400
BBa_I13621	.	engineered_region	2180	2220	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13621	.	stop_codon	2213	2213	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13621	.	polyA_site	2207	2220	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13621	.	stem_loop	2187	2206	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13621	.	terminator	2092	2171	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q01400
BBa_I13621	.	engineered_region	2092	2171	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13621	.	stem_loop	2103	2146	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13621	.	promoter	2229	2282	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q01400
BBa_I13621	.	non_covalent_binding_site	2254	2272	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13621	.	non_covalent_binding_site	2229	2247	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13621	.	engineered_region	2229	2282	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13621	.	promoter	2271	2276	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13621	.	promoter	2248	2253	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_I13621
tcacacaggactactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatca
gaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggag
ctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtc
tggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtc
gatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatc
attaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatg
tctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgc
attgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcat
aaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaa
ccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcg
cgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgt
tatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactct
ctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatac
gcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattg
tgagcggataacaagatactgagcacatactagagtcacacaggaaacctactagatgtccagattagataaaagtaaa
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctag
gtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttaga
taggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttaga
tgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcg
aaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattt
tactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatg
ccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattga
tcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctta
ataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac