LOCUS       BBa_I13623              2282 bp    DNA     linear       22-Dec-2024
DEFINITION  Cascaded Lac (medium) and Tet (weak) QPIs
ACCESSION   BBa_I13623
VERSION     BBa_I13623.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     misc_feature    1110..1148
                     /label=LVA
     CDS             21..1148
                     /label=lacI-LVA
     misc_feature    21..1148
                     /label=BBa_C0012
     misc_feature    1149..1173
                     /label=Help:Barcodes
     CDS             21..1148
                     /label=BBa_C0012
     stem_loop       1193..1236
                     /label=stem_loop
     misc_feature    1182..1261
                     /label=BBa_B0010
     terminator      1182..1261
                     /label=BBa_B0010
     stem_loop       1277..1296
                     /label=T7 TE
     misc_feature    1303..1303
                     /label=stop
     polyA_site      1297..1310
                     /label=polya
     misc_feature    1270..1310
                     /label=BBa_B0012
     terminator      1270..1310
                     /label=BBa_B0012
     terminator      1182..1310
                     /label=BBa_B0015
     misc_feature    1321..1337
                     /label=lac O1
     promoter        1338..1343
                     /label=-35
     misc_feature    1344..1360
                     /label=lac O1
     promoter        1361..1366
                     /label=-10
     misc_feature    1319..1372
                     /label=BBa_R0011
     promoter        1319..1372
                     /label=BBa_R0011
     misc_feature    1..1373
                     /label=BBa_Q01121
     RBS             1382..1392
                     /label=RBS-4\Weaker
     RBS             1388..1391
                     /label=RBS
     misc_feature    1382..1392
                     /label=BBa_B0033
     RBS             1382..1392
                     /label=BBa_B0033
     CDS             1402..2018
                     /label=tetR
     misc_feature    2019..2052
                     /label=SsrA
     misc_feature    2059..2083
                     /label=Help:Barcodes
     CDS             1399..2058
                     /label=BBa_C0040
     stem_loop       2103..2146
                     /label=stem_loop
     misc_feature    2092..2171
                     /label=BBa_B0010
     terminator      2092..2171
                     /label=BBa_B0010
     stem_loop       2187..2206
                     /label=T7 TE
     misc_feature    2213..2213
                     /label=stop
     polyA_site      2207..2220
                     /label=polya
     misc_feature    2180..2220
                     /label=BBa_B0012
     terminator      2180..2220
                     /label=BBa_B0012
     misc_feature    2229..2247
                     /label=TetR 1
     misc_feature    2229..2282
                     /label=BBa_R0040
     promoter        2248..2253
                     /label=-35
     misc_feature    2254..2272
                     /label=TetR 2
     promoter        2271..2276
                     /label=-10
     promoter        2229..2282
                     /label=BBa_R0040
     misc_feature    1382..2282
                     /label=BBa_Q03400
ORIGIN
        1 tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
       61 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      121 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      181 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      241 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      301 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      361 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      421 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      481 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      541 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      601 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      661 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      721 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      781 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      841 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      901 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
      961 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1021 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1081 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1141 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1321 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1381 gtcacacagg actactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
     1441 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
     1501 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
     1561 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
     1621 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
     1681 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
     1741 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
     1801 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
     1861 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
     1921 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
     1981 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
     2041 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
     2101 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2161 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2221 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     2281 ac
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