LOCUS       BBa_I13624              2285 bp    DNA     linear       17-May-2024
DEFINITION  Cascaded Lac (medium) and Tet (medium) QPIs
ACCESSION   BBa_I13624
VERSION     BBa_I13624.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     misc_feature    1110..1148
                     /label=LVA
     CDS             21..1148
                     /label=lacI-LVA
     misc_feature    21..1148
                     /label=BBa_C0012
     misc_feature    1149..1173
                     /label=Help:Barcodes
     CDS             21..1148
                     /label=BBa_C0012
     stem_loop       1193..1236
                     /label=stem_loop
     misc_feature    1182..1261
                     /label=BBa_B0010
     terminator      1182..1261
                     /label=BBa_B0010
     stem_loop       1277..1296
                     /label=T7 TE
     misc_feature    1303..1303
                     /label=stop
     polyA_site      1297..1310
                     /label=polya
     misc_feature    1270..1310
                     /label=BBa_B0012
     terminator      1270..1310
                     /label=BBa_B0012
     terminator      1182..1310
                     /label=BBa_B0015
     misc_feature    1321..1337
                     /label=lac O1
     promoter        1338..1343
                     /label=-35
     misc_feature    1344..1360
                     /label=lac O1
     promoter        1361..1366
                     /label=-10
     misc_feature    1319..1372
                     /label=BBa_R0011
     promoter        1319..1372
                     /label=BBa_R0011
     misc_feature    1..1373
                     /label=BBa_Q01121
     misc_feature    1388..1391
                     /label=conserved
     RBS             1382..1395
                     /label=BBa_B0031
     CDS             1405..2021
                     /label=tetR
     misc_feature    2022..2055
                     /label=SsrA
     misc_feature    2062..2086
                     /label=Help:Barcodes
     CDS             1402..2061
                     /label=BBa_C0040
     stem_loop       2106..2149
                     /label=stem_loop
     misc_feature    2095..2174
                     /label=BBa_B0010
     terminator      2095..2174
                     /label=BBa_B0010
     stem_loop       2190..2209
                     /label=T7 TE
     misc_feature    2216..2216
                     /label=stop
     polyA_site      2210..2223
                     /label=polya
     misc_feature    2183..2223
                     /label=BBa_B0012
     terminator      2183..2223
                     /label=BBa_B0012
     misc_feature    2232..2250
                     /label=TetR 1
     misc_feature    2232..2285
                     /label=BBa_R0040
     promoter        2251..2256
                     /label=-35
     misc_feature    2257..2275
                     /label=TetR 2
     promoter        2274..2279
                     /label=-10
     promoter        2232..2285
                     /label=BBa_R0040
     misc_feature    1382..2285
                     /label=BBa_Q01400
ORIGIN
        1 tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
       61 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      121 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      181 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      241 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      301 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      361 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      421 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      481 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      541 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      601 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      661 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      721 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      781 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      841 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      901 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
      961 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1021 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1081 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1141 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1321 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1381 gtcacacagg aaacctacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
     1441 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
     1501 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
     1561 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
     1621 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
     1681 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
     1741 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
     1801 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
     1861 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
     1921 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
     1981 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
     2041 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
     2101 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2161 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2221 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg
     2281 agcac
//