LOCUS       BBa_I13625              2283 bp    DNA     linear       25-Nov-2024
DEFINITION  Cascaded Lac (medium) and Tet (elowitz) QPIs
ACCESSION   BBa_I13625
VERSION     BBa_I13625.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     misc_feature    1110..1148
                     /label=LVA
     CDS             21..1148
                     /label=lacI-LVA
     misc_feature    21..1148
                     /label=BBa_C0012
     misc_feature    1149..1173
                     /label=Help:Barcodes
     CDS             21..1148
                     /label=BBa_C0012
     stem_loop       1193..1236
                     /label=stem_loop
     misc_feature    1182..1261
                     /label=BBa_B0010
     terminator      1182..1261
                     /label=BBa_B0010
     stem_loop       1277..1296
                     /label=T7 TE
     misc_feature    1303..1303
                     /label=stop
     polyA_site      1297..1310
                     /label=polya
     misc_feature    1270..1310
                     /label=BBa_B0012
     terminator      1270..1310
                     /label=BBa_B0012
     terminator      1182..1310
                     /label=BBa_B0015
     misc_feature    1321..1337
                     /label=lac O1
     promoter        1338..1343
                     /label=-35
     misc_feature    1344..1360
                     /label=lac O1
     promoter        1361..1366
                     /label=-10
     misc_feature    1319..1372
                     /label=BBa_R0011
     promoter        1319..1372
                     /label=BBa_R0011
     misc_feature    1..1373
                     /label=BBa_Q01121
     misc_feature    1386..1389
                     /label=conserved
     RBS             1382..1393
                     /label=BBa_B0034
     CDS             1403..2019
                     /label=tetR
     misc_feature    2020..2053
                     /label=SsrA
     misc_feature    2060..2084
                     /label=Help:Barcodes
     CDS             1400..2059
                     /label=BBa_C0040
     stem_loop       2104..2147
                     /label=stem_loop
     misc_feature    2093..2172
                     /label=BBa_B0010
     terminator      2093..2172
                     /label=BBa_B0010
     stem_loop       2188..2207
                     /label=T7 TE
     misc_feature    2214..2214
                     /label=stop
     polyA_site      2208..2221
                     /label=polya
     misc_feature    2181..2221
                     /label=BBa_B0012
     terminator      2181..2221
                     /label=BBa_B0012
     misc_feature    2230..2248
                     /label=TetR 1
     misc_feature    2230..2283
                     /label=BBa_R0040
     promoter        2249..2254
                     /label=-35
     misc_feature    2255..2273
                     /label=TetR 2
     promoter        2272..2277
                     /label=-10
     promoter        2230..2283
                     /label=BBa_R0040
     misc_feature    1382..2283
                     /label=BBa_Q04400
ORIGIN
        1 tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
       61 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      121 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      181 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      241 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      301 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      361 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      421 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      481 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      541 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      601 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      661 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      721 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      781 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      841 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      901 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
      961 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1021 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1081 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1141 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1321 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1381 gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
     1441 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
     1501 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
     1561 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
     1621 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
     1681 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
     1741 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
     1801 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
     1861 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
     1921 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
     1981 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
     2041 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat
     2101 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2161 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2221 atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag
     2281 cac
//