LOCUS       BBa_I13627              2283 bp    DNA     linear       29-Nov-2024
DEFINITION  Cascaded Lac (elowitz) and Tet (medium) QPIs
ACCESSION   BBa_I13627
VERSION     BBa_I13627.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     terminator      1180..1308
                     /label=BBa_B0015
     misc_feature    1319..1335
                     /label=lac O1
     promoter        1336..1341
                     /label=-35
     misc_feature    1342..1358
                     /label=lac O1
     promoter        1359..1364
                     /label=-10
     misc_feature    1317..1370
                     /label=BBa_R0011
     promoter        1317..1370
                     /label=BBa_R0011
     misc_feature    1..1371
                     /label=BBa_Q04121
     misc_feature    1386..1389
                     /label=conserved
     RBS             1380..1393
                     /label=BBa_B0031
     CDS             1403..2019
                     /label=tetR
     misc_feature    2020..2053
                     /label=SsrA
     misc_feature    2060..2084
                     /label=Help:Barcodes
     CDS             1400..2059
                     /label=BBa_C0040
     stem_loop       2104..2147
                     /label=stem_loop
     misc_feature    2093..2172
                     /label=BBa_B0010
     terminator      2093..2172
                     /label=BBa_B0010
     stem_loop       2188..2207
                     /label=T7 TE
     misc_feature    2214..2214
                     /label=stop
     polyA_site      2208..2221
                     /label=polya
     misc_feature    2181..2221
                     /label=BBa_B0012
     terminator      2181..2221
                     /label=BBa_B0012
     misc_feature    2230..2248
                     /label=TetR 1
     misc_feature    2230..2283
                     /label=BBa_R0040
     promoter        2249..2254
                     /label=-35
     misc_feature    2255..2273
                     /label=TetR 2
     promoter        2272..2277
                     /label=-10
     promoter        2230..2283
                     /label=BBa_R0040
     misc_feature    1380..2283
                     /label=BBa_Q01400
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt
     1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagagt
     1381 cacacaggaa acctactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
     1441 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
     1501 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
     1561 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
     1621 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
     1681 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
     1741 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
     1801 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
     1861 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
     1921 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
     1981 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
     2041 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat
     2101 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2161 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2221 atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag
     2281 cac
//