LOCUS BBa_I13631 2419 bp DNA linear 21-May-2024 DEFINITION Cascaded Tet (weak) and Lac (medium) QPIs with double terminator as input ACCESSION BBa_I13631 VERSION BBa_I13631.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 RBS 138..148 /label=RBS-4\Weaker RBS 144..147 /label=RBS misc_feature 138..148 /label=BBa_B0033 RBS 138..148 /label=BBa_B0033 CDS 158..774 /label=tetR misc_feature 775..808 /label=SsrA misc_feature 815..839 /label=Help:Barcodes CDS 155..814 /label=BBa_C0040 stem_loop 859..902 /label=stem_loop misc_feature 848..927 /label=BBa_B0010 terminator 848..927 /label=BBa_B0010 stem_loop 943..962 /label=T7 TE misc_feature 969..969 /label=stop polyA_site 963..976 /label=polya misc_feature 936..976 /label=BBa_B0012 terminator 936..976 /label=BBa_B0012 misc_feature 985..1003 /label=TetR 1 misc_feature 985..1038 /label=BBa_R0040 promoter 1004..1009 /label=-35 misc_feature 1010..1028 /label=TetR 2 promoter 1027..1032 /label=-10 promoter 985..1038 /label=BBa_R0040 misc_feature 138..1038 /label=BBa_Q03400 misc_feature 1053..1056 /label=conserved RBS 1047..1060 /label=BBa_B0031 misc_feature 2156..2194 /label=LVA CDS 1067..2194 /label=lacI-LVA misc_feature 1067..2194 /label=BBa_C0012 misc_feature 2195..2219 /label=Help:Barcodes CDS 1067..2194 /label=BBa_C0012 stem_loop 2239..2282 /label=stem_loop misc_feature 2228..2307 /label=BBa_B0010 terminator 2228..2307 /label=BBa_B0010 stem_loop 2323..2342 /label=T7 TE misc_feature 2349..2349 /label=stop polyA_site 2343..2356 /label=polya misc_feature 2316..2356 /label=BBa_B0012 terminator 2316..2356 /label=BBa_B0012 terminator 2228..2356 /label=BBa_B0015 misc_feature 2367..2383 /label=lac O1 promoter 2384..2389 /label=-35 misc_feature 2390..2406 /label=lac O1 promoter 2407..2412 /label=-10 misc_feature 2365..2418 /label=BBa_R0011 promoter 2365..2418 /label=BBa_R0011 misc_feature 1047..2419 /label=BBa_Q01121 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagtca cacaggacta ctagatgtcc agattagata aaagtaaagt 181 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa 241 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg 301 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc 361 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc 421 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca 481 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa 541 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca 601 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt 661 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg 721 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc 781 aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact 841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 961 cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt 1021 gatagagata ctgagcacta ctagagtcac acaggaaacc tactagatgg tgaatgtgaa 1081 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 1141 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 1201 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt 1261 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc 1321 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 1381 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 1441 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 1501 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 1561 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 1621 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 1681 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 1741 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 1801 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 1861 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta 1921 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 1981 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 2041 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 2101 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc 2161 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct 2221 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 2281 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 2341 cctttctgcg tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga 2401 taacaagata ctgagcaca //