LOCUS BBa_I13633 2419 bp DNA linear 21-May-2024 DEFINITION Cascaded Tet (medium) and Lac (weak) QPIs with double terminator as input ACCESSION BBa_I13633 VERSION BBa_I13633.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 144..147 /label=conserved RBS 138..151 /label=BBa_B0031 CDS 161..777 /label=tetR misc_feature 778..811 /label=SsrA misc_feature 818..842 /label=Help:Barcodes CDS 158..817 /label=BBa_C0040 stem_loop 862..905 /label=stem_loop misc_feature 851..930 /label=BBa_B0010 terminator 851..930 /label=BBa_B0010 stem_loop 946..965 /label=T7 TE misc_feature 972..972 /label=stop polyA_site 966..979 /label=polya misc_feature 939..979 /label=BBa_B0012 terminator 939..979 /label=BBa_B0012 misc_feature 988..1006 /label=TetR 1 misc_feature 988..1041 /label=BBa_R0040 promoter 1007..1012 /label=-35 misc_feature 1013..1031 /label=TetR 2 promoter 1030..1035 /label=-10 promoter 988..1041 /label=BBa_R0040 misc_feature 138..1041 /label=BBa_Q01400 RBS 1050..1060 /label=RBS-4\Weaker RBS 1056..1059 /label=RBS misc_feature 1050..1060 /label=BBa_B0033 RBS 1050..1060 /label=BBa_B0033 misc_feature 2156..2194 /label=LVA CDS 1067..2194 /label=lacI-LVA misc_feature 1067..2194 /label=BBa_C0012 misc_feature 2195..2219 /label=Help:Barcodes CDS 1067..2194 /label=BBa_C0012 stem_loop 2239..2282 /label=stem_loop misc_feature 2228..2307 /label=BBa_B0010 terminator 2228..2307 /label=BBa_B0010 stem_loop 2323..2342 /label=T7 TE misc_feature 2349..2349 /label=stop polyA_site 2343..2356 /label=polya misc_feature 2316..2356 /label=BBa_B0012 terminator 2316..2356 /label=BBa_B0012 terminator 2228..2356 /label=BBa_B0015 misc_feature 2367..2383 /label=lac O1 promoter 2384..2389 /label=-35 misc_feature 2390..2406 /label=lac O1 promoter 2407..2412 /label=-10 misc_feature 2365..2418 /label=BBa_R0011 promoter 2365..2418 /label=BBa_R0011 misc_feature 1050..2419 /label=BBa_Q03121 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagtca cacaggaaac ctactagatg tccagattag ataaaagtaa 181 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg 241 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa 301 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg 361 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg 421 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa 481 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga 541 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga 601 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc 661 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt 721 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc 781 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 961 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc 1021 agtgatagag atactgagca ctactagagt cacacaggac tactagatgg tgaatgtgaa 1081 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 1141 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 1201 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt 1261 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc 1321 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 1381 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 1441 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 1501 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 1561 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 1621 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 1681 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 1741 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 1801 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 1861 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta 1921 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 1981 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 2041 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 2101 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc 2161 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct 2221 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 2281 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 2341 cctttctgcg tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga 2401 taacaagata ctgagcaca //