LOCUS       BBa_I13634              2422 bp    DNA     linear       21-May-2024
DEFINITION  Cascaded Tet (medium) and Lac (medium) QPIs with double terminator 
            as input
ACCESSION   BBa_I13634
VERSION     BBa_I13634.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    144..147
                     /label=conserved
     RBS             138..151
                     /label=BBa_B0031
     CDS             161..777
                     /label=tetR
     misc_feature    778..811
                     /label=SsrA
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             158..817
                     /label=BBa_C0040
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    988..1006
                     /label=TetR 1
     misc_feature    988..1041
                     /label=BBa_R0040
     promoter        1007..1012
                     /label=-35
     misc_feature    1013..1031
                     /label=TetR 2
     promoter        1030..1035
                     /label=-10
     promoter        988..1041
                     /label=BBa_R0040
     misc_feature    138..1041
                     /label=BBa_Q01400
     misc_feature    1056..1059
                     /label=conserved
     RBS             1050..1063
                     /label=BBa_B0031
     misc_feature    2159..2197
                     /label=LVA
     CDS             1070..2197
                     /label=lacI-LVA
     misc_feature    1070..2197
                     /label=BBa_C0012
     misc_feature    2198..2222
                     /label=Help:Barcodes
     CDS             1070..2197
                     /label=BBa_C0012
     stem_loop       2242..2285
                     /label=stem_loop
     misc_feature    2231..2310
                     /label=BBa_B0010
     terminator      2231..2310
                     /label=BBa_B0010
     stem_loop       2326..2345
                     /label=T7 TE
     misc_feature    2352..2352
                     /label=stop
     polyA_site      2346..2359
                     /label=polya
     misc_feature    2319..2359
                     /label=BBa_B0012
     terminator      2319..2359
                     /label=BBa_B0012
     terminator      2231..2359
                     /label=BBa_B0015
     misc_feature    2370..2386
                     /label=lac O1
     promoter        2387..2392
                     /label=-35
     misc_feature    2393..2409
                     /label=lac O1
     promoter        2410..2415
                     /label=-10
     misc_feature    2368..2421
                     /label=BBa_R0011
     promoter        2368..2421
                     /label=BBa_R0011
     misc_feature    1050..2422
                     /label=BBa_Q01121
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtca cacaggaaac ctactagatg tccagattag ataaaagtaa
      181 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
      241 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
      301 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
      361 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
      421 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
      481 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
      541 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
      601 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
      661 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
      721 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
      781 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc
     1021 agtgatagag atactgagca ctactagagt cacacaggaa acctactaga tggtgaatgt
     1081 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
     1141 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
     1201 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
     1261 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
     1321 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
     1381 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
     1441 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
     1501 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
     1561 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
     1621 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
     1681 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
     1741 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
     1801 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
     1861 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg
     1921 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
     1981 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
     2041 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
     2101 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc
     2161 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc
     2221 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2281 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2341 gggcctttct gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc
     2401 ggataacaag atactgagca ca
//