LOCUS       BBa_I13637              2420 bp    DNA     linear       21-May-2024
DEFINITION  Cascaded Tet (elowitz) and Lac (medium) QPIs with double 
            terminator as input
ACCESSION   BBa_I13637
VERSION     BBa_I13637.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    142..145
                     /label=conserved
     RBS             138..149
                     /label=BBa_B0034
     CDS             159..775
                     /label=tetR
     misc_feature    776..809
                     /label=SsrA
     misc_feature    816..840
                     /label=Help:Barcodes
     CDS             156..815
                     /label=BBa_C0040
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
     misc_feature    986..1004
                     /label=TetR 1
     misc_feature    986..1039
                     /label=BBa_R0040
     promoter        1005..1010
                     /label=-35
     misc_feature    1011..1029
                     /label=TetR 2
     promoter        1028..1033
                     /label=-10
     promoter        986..1039
                     /label=BBa_R0040
     misc_feature    138..1039
                     /label=BBa_Q04400
     misc_feature    1054..1057
                     /label=conserved
     RBS             1048..1061
                     /label=BBa_B0031
     misc_feature    2157..2195
                     /label=LVA
     CDS             1068..2195
                     /label=lacI-LVA
     misc_feature    1068..2195
                     /label=BBa_C0012
     misc_feature    2196..2220
                     /label=Help:Barcodes
     CDS             1068..2195
                     /label=BBa_C0012
     stem_loop       2240..2283
                     /label=stem_loop
     misc_feature    2229..2308
                     /label=BBa_B0010
     terminator      2229..2308
                     /label=BBa_B0010
     stem_loop       2324..2343
                     /label=T7 TE
     misc_feature    2350..2350
                     /label=stop
     polyA_site      2344..2357
                     /label=polya
     misc_feature    2317..2357
                     /label=BBa_B0012
     terminator      2317..2357
                     /label=BBa_B0012
     terminator      2229..2357
                     /label=BBa_B0015
     misc_feature    2368..2384
                     /label=lac O1
     promoter        2385..2390
                     /label=-35
     misc_feature    2391..2407
                     /label=lac O1
     promoter        2408..2413
                     /label=-10
     misc_feature    2366..2419
                     /label=BBa_R0011
     promoter        2366..2419
                     /label=BBa_R0011
     misc_feature    1048..2420
                     /label=BBa_Q01121
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag
      181 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
      241 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
      301 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
      361 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
      421 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
      481 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
      541 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
      601 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
      661 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
      721 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg
      781 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag
     1021 tgatagagat actgagcact actagagtca cacaggaaac ctactagatg gtgaatgtga
     1081 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
     1141 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
     1201 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     1261 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     1321 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     1381 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     1441 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     1501 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     1561 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     1621 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     1681 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     1741 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
     1801 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     1861 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     1921 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     1981 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     2041 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
     2101 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg
     2161 caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc
     2221 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2281 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2341 gcctttctgc gtttatatac tagagaattg tgagcggata acaattgaca ttgtgagcgg
     2401 ataacaagat actgagcaca
//