LOCUS BBa_I13644 2422 bp DNA linear 22-Dec-2024 DEFINITION Cascaded Lac (medium) and Tet (medium) QPIs with double terminator as input ACCESSION BBa_I13644 VERSION BBa_I13644.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 144..147 /label=conserved RBS 138..151 /label=BBa_B0031 misc_feature 1247..1285 /label=LVA CDS 158..1285 /label=lacI-LVA misc_feature 158..1285 /label=BBa_C0012 misc_feature 1286..1310 /label=Help:Barcodes CDS 158..1285 /label=BBa_C0012 stem_loop 1330..1373 /label=stem_loop misc_feature 1319..1398 /label=BBa_B0010 terminator 1319..1398 /label=BBa_B0010 stem_loop 1414..1433 /label=T7 TE misc_feature 1440..1440 /label=stop polyA_site 1434..1447 /label=polya misc_feature 1407..1447 /label=BBa_B0012 terminator 1407..1447 /label=BBa_B0012 terminator 1319..1447 /label=BBa_B0015 misc_feature 1458..1474 /label=lac O1 promoter 1475..1480 /label=-35 misc_feature 1481..1497 /label=lac O1 promoter 1498..1503 /label=-10 misc_feature 1456..1509 /label=BBa_R0011 promoter 1456..1509 /label=BBa_R0011 misc_feature 138..1510 /label=BBa_Q01121 misc_feature 1525..1528 /label=conserved RBS 1519..1532 /label=BBa_B0031 CDS 1542..2158 /label=tetR misc_feature 2159..2192 /label=SsrA misc_feature 2199..2223 /label=Help:Barcodes CDS 1539..2198 /label=BBa_C0040 stem_loop 2243..2286 /label=stem_loop misc_feature 2232..2311 /label=BBa_B0010 terminator 2232..2311 /label=BBa_B0010 stem_loop 2327..2346 /label=T7 TE misc_feature 2353..2353 /label=stop polyA_site 2347..2360 /label=polya misc_feature 2320..2360 /label=BBa_B0012 terminator 2320..2360 /label=BBa_B0012 misc_feature 2369..2387 /label=TetR 1 misc_feature 2369..2422 /label=BBa_R0040 promoter 2388..2393 /label=-35 misc_feature 2394..2412 /label=TetR 2 promoter 2411..2416 /label=-10 promoter 2369..2422 /label=BBa_R0040 misc_feature 1519..2422 /label=BBa_Q01400 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagtca cacaggaaac ctactagatg gtgaatgtga aaccagtaac 181 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 241 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 301 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 361 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 421 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 481 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 541 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 601 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 661 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 721 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 781 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 841 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 901 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 961 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 1021 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 1081 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 1141 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 1201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga 1261 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc 1321 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1381 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1441 gtttatatac tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat 1501 actgagcaca tactagagtc acacaggaaa cctactagat gtccagatta gataaaagta 1561 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc 1621 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata 1681 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt 1741 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat 1801 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa 1861 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag 1921 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag 1981 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc 2041 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat 2101 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg 2161 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat 2221 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 2281 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 2341 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat 2401 cagtgataga gatactgagc ac //