LOCUS       BBa_I13644              2422 bp    DNA     linear       23-May-2024
DEFINITION  Cascaded Lac (medium) and Tet (medium) QPIs with double terminator 
            as input
ACCESSION   BBa_I13644
VERSION     BBa_I13644.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    144..147
                     /label=conserved
     RBS             138..151
                     /label=BBa_B0031
     misc_feature    1247..1285
                     /label=LVA
     CDS             158..1285
                     /label=lacI-LVA
     misc_feature    158..1285
                     /label=BBa_C0012
     misc_feature    1286..1310
                     /label=Help:Barcodes
     CDS             158..1285
                     /label=BBa_C0012
     stem_loop       1330..1373
                     /label=stem_loop
     misc_feature    1319..1398
                     /label=BBa_B0010
     terminator      1319..1398
                     /label=BBa_B0010
     stem_loop       1414..1433
                     /label=T7 TE
     misc_feature    1440..1440
                     /label=stop
     polyA_site      1434..1447
                     /label=polya
     misc_feature    1407..1447
                     /label=BBa_B0012
     terminator      1407..1447
                     /label=BBa_B0012
     terminator      1319..1447
                     /label=BBa_B0015
     misc_feature    1458..1474
                     /label=lac O1
     promoter        1475..1480
                     /label=-35
     misc_feature    1481..1497
                     /label=lac O1
     promoter        1498..1503
                     /label=-10
     misc_feature    1456..1509
                     /label=BBa_R0011
     promoter        1456..1509
                     /label=BBa_R0011
     misc_feature    138..1510
                     /label=BBa_Q01121
     misc_feature    1525..1528
                     /label=conserved
     RBS             1519..1532
                     /label=BBa_B0031
     CDS             1542..2158
                     /label=tetR
     misc_feature    2159..2192
                     /label=SsrA
     misc_feature    2199..2223
                     /label=Help:Barcodes
     CDS             1539..2198
                     /label=BBa_C0040
     stem_loop       2243..2286
                     /label=stem_loop
     misc_feature    2232..2311
                     /label=BBa_B0010
     terminator      2232..2311
                     /label=BBa_B0010
     stem_loop       2327..2346
                     /label=T7 TE
     misc_feature    2353..2353
                     /label=stop
     polyA_site      2347..2360
                     /label=polya
     misc_feature    2320..2360
                     /label=BBa_B0012
     terminator      2320..2360
                     /label=BBa_B0012
     misc_feature    2369..2387
                     /label=TetR 1
     misc_feature    2369..2422
                     /label=BBa_R0040
     promoter        2388..2393
                     /label=-35
     misc_feature    2394..2412
                     /label=TetR 2
     promoter        2411..2416
                     /label=-10
     promoter        2369..2422
                     /label=BBa_R0040
     misc_feature    1519..2422
                     /label=BBa_Q01400
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtca cacaggaaac ctactagatg gtgaatgtga aaccagtaac
      181 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
      241 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
      301 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
      361 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
      421 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
      481 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
      541 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
      601 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
      661 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
      721 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
      781 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg
      841 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
      901 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
      961 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     1021 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     1081 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     1141 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga
     1261 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc
     1321 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1381 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1441 gtttatatac tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat
     1501 actgagcaca tactagagtc acacaggaaa cctactagat gtccagatta gataaaagta
     1561 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc
     1621 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata
     1681 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt
     1741 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat
     1801 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa
     1861 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag
     1921 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag
     1981 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc
     2041 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat
     2101 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg
     2161 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat
     2221 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2281 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2341 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat
     2401 cagtgataga gatactgagc ac
//