LOCUS BBa_I13645 2420 bp DNA linear 21-May-2024 DEFINITION Cascaded Lac (medium) and Tet (elowitz) QPIs with double terminator as input ACCESSION BBa_I13645 VERSION BBa_I13645.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 144..147 /label=conserved RBS 138..151 /label=BBa_B0031 misc_feature 1247..1285 /label=LVA CDS 158..1285 /label=lacI-LVA misc_feature 158..1285 /label=BBa_C0012 misc_feature 1286..1310 /label=Help:Barcodes CDS 158..1285 /label=BBa_C0012 stem_loop 1330..1373 /label=stem_loop misc_feature 1319..1398 /label=BBa_B0010 terminator 1319..1398 /label=BBa_B0010 stem_loop 1414..1433 /label=T7 TE misc_feature 1440..1440 /label=stop polyA_site 1434..1447 /label=polya misc_feature 1407..1447 /label=BBa_B0012 terminator 1407..1447 /label=BBa_B0012 terminator 1319..1447 /label=BBa_B0015 misc_feature 1458..1474 /label=lac O1 promoter 1475..1480 /label=-35 misc_feature 1481..1497 /label=lac O1 promoter 1498..1503 /label=-10 misc_feature 1456..1509 /label=BBa_R0011 promoter 1456..1509 /label=BBa_R0011 misc_feature 138..1510 /label=BBa_Q01121 misc_feature 1523..1526 /label=conserved RBS 1519..1530 /label=BBa_B0034 CDS 1540..2156 /label=tetR misc_feature 2157..2190 /label=SsrA misc_feature 2197..2221 /label=Help:Barcodes CDS 1537..2196 /label=BBa_C0040 stem_loop 2241..2284 /label=stem_loop misc_feature 2230..2309 /label=BBa_B0010 terminator 2230..2309 /label=BBa_B0010 stem_loop 2325..2344 /label=T7 TE misc_feature 2351..2351 /label=stop polyA_site 2345..2358 /label=polya misc_feature 2318..2358 /label=BBa_B0012 terminator 2318..2358 /label=BBa_B0012 misc_feature 2367..2385 /label=TetR 1 misc_feature 2367..2420 /label=BBa_R0040 promoter 2386..2391 /label=-35 misc_feature 2392..2410 /label=TetR 2 promoter 2409..2414 /label=-10 promoter 2367..2420 /label=BBa_R0040 misc_feature 1519..2420 /label=BBa_Q04400 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagtca cacaggaaac ctactagatg gtgaatgtga aaccagtaac 181 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 241 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 301 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 361 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 421 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 481 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 541 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 601 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 661 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 721 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 781 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 841 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 901 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 961 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 1021 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 1081 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 1141 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 1201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga 1261 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc 1321 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1381 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1441 gtttatatac tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat 1501 actgagcaca tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa 1561 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt 1621 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag 1681 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc 1741 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt 1801 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa 1861 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag 1921 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat 1981 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca 2041 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc 2101 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct 2161 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 2221 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2281 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2341 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca 2401 gtgatagaga tactgagcac //