LOCUS       BBa_I13647              2420 bp    DNA     linear       23-May-2024
DEFINITION  Cascaded Lac (elowitz) and Tet (medium) QPIs with double 
            terminator as input
ACCESSION   BBa_I13647
VERSION     BBa_I13647.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    142..145
                     /label=conserved
     RBS             138..149
                     /label=BBa_B0034
     misc_feature    1245..1283
                     /label=LVA
     CDS             156..1283
                     /label=lacI-LVA
     misc_feature    156..1283
                     /label=BBa_C0012
     misc_feature    1284..1308
                     /label=Help:Barcodes
     CDS             156..1283
                     /label=BBa_C0012
     stem_loop       1328..1371
                     /label=stem_loop
     misc_feature    1317..1396
                     /label=BBa_B0010
     terminator      1317..1396
                     /label=BBa_B0010
     stem_loop       1412..1431
                     /label=T7 TE
     misc_feature    1438..1438
                     /label=stop
     polyA_site      1432..1445
                     /label=polya
     misc_feature    1405..1445
                     /label=BBa_B0012
     terminator      1405..1445
                     /label=BBa_B0012
     terminator      1317..1445
                     /label=BBa_B0015
     misc_feature    1456..1472
                     /label=lac O1
     promoter        1473..1478
                     /label=-35
     misc_feature    1479..1495
                     /label=lac O1
     promoter        1496..1501
                     /label=-10
     misc_feature    1454..1507
                     /label=BBa_R0011
     promoter        1454..1507
                     /label=BBa_R0011
     misc_feature    138..1508
                     /label=BBa_Q04121
     misc_feature    1523..1526
                     /label=conserved
     RBS             1517..1530
                     /label=BBa_B0031
     CDS             1540..2156
                     /label=tetR
     misc_feature    2157..2190
                     /label=SsrA
     misc_feature    2197..2221
                     /label=Help:Barcodes
     CDS             1537..2196
                     /label=BBa_C0040
     stem_loop       2241..2284
                     /label=stem_loop
     misc_feature    2230..2309
                     /label=BBa_B0010
     terminator      2230..2309
                     /label=BBa_B0010
     stem_loop       2325..2344
                     /label=T7 TE
     misc_feature    2351..2351
                     /label=stop
     polyA_site      2345..2358
                     /label=polya
     misc_feature    2318..2358
                     /label=BBa_B0012
     terminator      2318..2358
                     /label=BBa_B0012
     misc_feature    2367..2385
                     /label=TetR 1
     misc_feature    2367..2420
                     /label=BBa_R0040
     promoter        2386..2391
                     /label=-35
     misc_feature    2392..2410
                     /label=TetR 2
     promoter        2409..2414
                     /label=-10
     promoter        2367..2420
                     /label=BBa_R0040
     misc_feature    1517..2420
                     /label=BBa_Q01400
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagaaa gaggagaaat actagatggt gaatgtgaaa ccagtaacgt
      181 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
      241 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
      301 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
      361 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
      421 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
      481 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
      541 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
      601 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
      661 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
      721 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
      781 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
      841 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
      901 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
      961 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     1021 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     1081 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     1141 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1201 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa
     1261 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag
     1321 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1381 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1441 ttatatacta gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac
     1501 tgagcacata ctagagtcac acaggaaacc tactagatgt ccagattaga taaaagtaaa
     1561 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
     1621 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
     1681 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
     1741 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
     1801 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
     1861 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
     1921 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
     1981 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
     2041 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
     2101 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct
     2161 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
     2221 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2281 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2341 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca
     2401 gtgatagaga tactgagcac
//