LOCUS       BBa_I13651              2339 bp    DNA     linear       23-May-2024
DEFINITION  Cascaded Tet (weak) and Lac (medium) QPIs with constitutive 
            promoter as input
ACCESSION   BBa_I13651
VERSION     BBa_I13651.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..68
                     /label=RBS-4\Weaker
     RBS             64..67
                     /label=RBS
     misc_feature    58..68
                     /label=BBa_B0033
     RBS             58..68
                     /label=BBa_B0033
     CDS             78..694
                     /label=tetR
     misc_feature    695..728
                     /label=SsrA
     misc_feature    735..759
                     /label=Help:Barcodes
     CDS             75..734
                     /label=BBa_C0040
     stem_loop       779..822
                     /label=stem_loop
     misc_feature    768..847
                     /label=BBa_B0010
     terminator      768..847
                     /label=BBa_B0010
     stem_loop       863..882
                     /label=T7 TE
     misc_feature    889..889
                     /label=stop
     polyA_site      883..896
                     /label=polya
     misc_feature    856..896
                     /label=BBa_B0012
     terminator      856..896
                     /label=BBa_B0012
     misc_feature    905..923
                     /label=TetR 1
     misc_feature    905..958
                     /label=BBa_R0040
     promoter        924..929
                     /label=-35
     misc_feature    930..948
                     /label=TetR 2
     promoter        947..952
                     /label=-10
     promoter        905..958
                     /label=BBa_R0040
     misc_feature    58..958
                     /label=BBa_Q03400
     misc_feature    973..976
                     /label=conserved
     RBS             967..980
                     /label=BBa_B0031
     misc_feature    2076..2114
                     /label=LVA
     CDS             987..2114
                     /label=lacI-LVA
     misc_feature    987..2114
                     /label=BBa_C0012
     misc_feature    2115..2139
                     /label=Help:Barcodes
     CDS             987..2114
                     /label=BBa_C0012
     stem_loop       2159..2202
                     /label=stem_loop
     misc_feature    2148..2227
                     /label=BBa_B0010
     terminator      2148..2227
                     /label=BBa_B0010
     stem_loop       2243..2262
                     /label=T7 TE
     misc_feature    2269..2269
                     /label=stop
     polyA_site      2263..2276
                     /label=polya
     misc_feature    2236..2276
                     /label=BBa_B0012
     terminator      2236..2276
                     /label=BBa_B0012
     terminator      2148..2276
                     /label=BBa_B0015
     misc_feature    2287..2303
                     /label=lac O1
     promoter        2304..2309
                     /label=-35
     misc_feature    2310..2326
                     /label=lac O1
     promoter        2327..2332
                     /label=-10
     misc_feature    2285..2338
                     /label=BBa_R0011
     promoter        2285..2338
                     /label=BBa_R0011
     misc_feature    967..2339
                     /label=BBa_Q01121
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggacta ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc
      121 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
      181 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
      241 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
      301 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
      361 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
      421 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
      481 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
      541 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
      601 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
      661 gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt
      721 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat
      781 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      841 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      901 agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta
      961 ctagagtcac acaggaaacc tactagatgg tgaatgtgaa accagtaacg ttatacgatg
     1021 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     1081 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     1141 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     1201 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     1261 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     1321 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     1381 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     1441 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     1501 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     1561 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     1621 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     1681 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     1741 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     1801 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     1861 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     1921 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     1981 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt
     2101 tagtagctta ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat
     2161 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2221 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
     2281 agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata ctgagcaca
//