LOCUS       BBa_I13653              2339 bp    DNA     linear       22-Dec-2024
DEFINITION  Cascaded Tet (medium) and Lac (weak) QPIs with constitutive 
            promoter as input
ACCESSION   BBa_I13653
VERSION     BBa_I13653.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    64..67
                     /label=conserved
     RBS             58..71
                     /label=BBa_B0031
     CDS             81..697
                     /label=tetR
     misc_feature    698..731
                     /label=SsrA
     misc_feature    738..762
                     /label=Help:Barcodes
     CDS             78..737
                     /label=BBa_C0040
     stem_loop       782..825
                     /label=stem_loop
     misc_feature    771..850
                     /label=BBa_B0010
     terminator      771..850
                     /label=BBa_B0010
     stem_loop       866..885
                     /label=T7 TE
     misc_feature    892..892
                     /label=stop
     polyA_site      886..899
                     /label=polya
     misc_feature    859..899
                     /label=BBa_B0012
     terminator      859..899
                     /label=BBa_B0012
     misc_feature    908..926
                     /label=TetR 1
     misc_feature    908..961
                     /label=BBa_R0040
     promoter        927..932
                     /label=-35
     misc_feature    933..951
                     /label=TetR 2
     promoter        950..955
                     /label=-10
     promoter        908..961
                     /label=BBa_R0040
     misc_feature    58..961
                     /label=BBa_Q01400
     RBS             970..980
                     /label=RBS-4\Weaker
     RBS             976..979
                     /label=RBS
     misc_feature    970..980
                     /label=BBa_B0033
     RBS             970..980
                     /label=BBa_B0033
     misc_feature    2076..2114
                     /label=LVA
     CDS             987..2114
                     /label=lacI-LVA
     misc_feature    987..2114
                     /label=BBa_C0012
     misc_feature    2115..2139
                     /label=Help:Barcodes
     CDS             987..2114
                     /label=BBa_C0012
     stem_loop       2159..2202
                     /label=stem_loop
     misc_feature    2148..2227
                     /label=BBa_B0010
     terminator      2148..2227
                     /label=BBa_B0010
     stem_loop       2243..2262
                     /label=T7 TE
     misc_feature    2269..2269
                     /label=stop
     polyA_site      2263..2276
                     /label=polya
     misc_feature    2236..2276
                     /label=BBa_B0012
     terminator      2236..2276
                     /label=BBa_B0012
     terminator      2148..2276
                     /label=BBa_B0015
     misc_feature    2287..2303
                     /label=lac O1
     promoter        2304..2309
                     /label=-35
     misc_feature    2310..2326
                     /label=lac O1
     promoter        2327..2332
                     /label=-10
     misc_feature    2285..2338
                     /label=BBa_R0011
     promoter        2285..2338
                     /label=BBa_R0011
     misc_feature    970..2339
                     /label=BBa_Q03121
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaac ctactagatg tccagattag ataaaagtaa agtgattaac agcgcattag
      121 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
      181 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
      241 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
      301 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
      361 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
      421 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
      481 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
      541 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
      601 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
      661 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg
      721 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca
      781 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      841 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      901 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
      961 ctactagagt cacacaggac tactagatgg tgaatgtgaa accagtaacg ttatacgatg
     1021 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     1081 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     1141 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     1201 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     1261 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     1321 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     1381 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     1441 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     1501 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     1561 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     1621 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     1681 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     1741 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     1801 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     1861 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     1921 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     1981 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt
     2101 tagtagctta ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat
     2161 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2221 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
     2281 agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata ctgagcaca
//