##gff-version 3
##sequence-region BBa_I13653 1 2339
BBa_I13653	.	engineered_region	970	2339	.	+	0	ID=BBa_Q03121;Name=BBa_Q03121
BBa_I13653	.	CDS	987	2114	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q03121
BBa_I13653	.	engineered_tag	2115	2139	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13653	.	CDS	987	2114	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13653	.	tag	2076	2114	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13653	.	engineered_region	987	2114	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13653	.	ribosome_entry_site	970	980	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_Q03121
BBa_I13653	.	engineered_region	970	980	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_I13653	.	ribosome_entry_site	970	980	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_I13653	.	ribosome_entry_site	976	979	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_I13653	.	promoter	2285	2338	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q03121
BBa_I13653	.	promoter	2327	2332	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13653	.	engineered_region	2285	2338	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13653	.	promoter	2304	2309	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13653	.	operator	2287	2303	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13653	.	operator	2310	2326	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13653	.	terminator	2148	2276	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q03121
BBa_I13653	.	terminator	2236	2276	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13653	.	engineered_region	2236	2276	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13653	.	stop_codon	2269	2269	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13653	.	polyA_site	2263	2276	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13653	.	stem_loop	2243	2262	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13653	.	terminator	2148	2227	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13653	.	engineered_region	2148	2227	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13653	.	stem_loop	2159	2202	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13653	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I13653	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I13653	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I13653	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I13653	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I13653	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I13653	.	engineered_region	58	961	.	+	0	ID=BBa_Q01400;Name=BBa_Q01400
BBa_I13653	.	ribosome_entry_site	58	71	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01400
BBa_I13653	.	conserved_region	64	67	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13653	.	CDS	78	737	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q01400
BBa_I13653	.	tag	698	731	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13653	.	engineered_tag	738	762	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13653	.	CDS	81	697	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13653	.	terminator	859	899	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q01400
BBa_I13653	.	engineered_region	859	899	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13653	.	stop_codon	892	892	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13653	.	polyA_site	886	899	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13653	.	stem_loop	866	885	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13653	.	terminator	771	850	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q01400
BBa_I13653	.	engineered_region	771	850	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13653	.	stem_loop	782	825	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13653	.	promoter	908	961	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q01400
BBa_I13653	.	non_covalent_binding_site	933	951	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13653	.	non_covalent_binding_site	908	926	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13653	.	engineered_region	908	961	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13653	.	promoter	950	955	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13653	.	promoter	927	932	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_I13653
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaacctactagat
gtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacc
cgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacg
ccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacg
taataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctaca
gaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatg
cactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggga
aacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcc
ttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatag
agatactgagcactactagagtcacacaggactactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcaga
gtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaa
gtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctga
ttggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaact
gggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcg
caacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatg
ttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgact
gggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgt
ctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtg
ccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatca
gatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgac
gataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcg
tggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaa
aaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtt
tcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgta
gatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtg
agcggataacaattgacattgtgagcggataacaagatactgagcaca