LOCUS       BBa_I13654              2342 bp    DNA     linear       22-Dec-2024
DEFINITION  Cascaded Tet (medium) and Lac (medium) QPIs with constitutive 
            promoter as input
ACCESSION   BBa_I13654
VERSION     BBa_I13654.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    64..67
                     /label=conserved
     RBS             58..71
                     /label=BBa_B0031
     CDS             81..697
                     /label=tetR
     misc_feature    698..731
                     /label=SsrA
     misc_feature    738..762
                     /label=Help:Barcodes
     CDS             78..737
                     /label=BBa_C0040
     stem_loop       782..825
                     /label=stem_loop
     misc_feature    771..850
                     /label=BBa_B0010
     terminator      771..850
                     /label=BBa_B0010
     stem_loop       866..885
                     /label=T7 TE
     misc_feature    892..892
                     /label=stop
     polyA_site      886..899
                     /label=polya
     misc_feature    859..899
                     /label=BBa_B0012
     terminator      859..899
                     /label=BBa_B0012
     misc_feature    908..926
                     /label=TetR 1
     misc_feature    908..961
                     /label=BBa_R0040
     promoter        927..932
                     /label=-35
     misc_feature    933..951
                     /label=TetR 2
     promoter        950..955
                     /label=-10
     promoter        908..961
                     /label=BBa_R0040
     misc_feature    58..961
                     /label=BBa_Q01400
     misc_feature    976..979
                     /label=conserved
     RBS             970..983
                     /label=BBa_B0031
     misc_feature    2079..2117
                     /label=LVA
     CDS             990..2117
                     /label=lacI-LVA
     misc_feature    990..2117
                     /label=BBa_C0012
     misc_feature    2118..2142
                     /label=Help:Barcodes
     CDS             990..2117
                     /label=BBa_C0012
     stem_loop       2162..2205
                     /label=stem_loop
     misc_feature    2151..2230
                     /label=BBa_B0010
     terminator      2151..2230
                     /label=BBa_B0010
     stem_loop       2246..2265
                     /label=T7 TE
     misc_feature    2272..2272
                     /label=stop
     polyA_site      2266..2279
                     /label=polya
     misc_feature    2239..2279
                     /label=BBa_B0012
     terminator      2239..2279
                     /label=BBa_B0012
     terminator      2151..2279
                     /label=BBa_B0015
     misc_feature    2290..2306
                     /label=lac O1
     promoter        2307..2312
                     /label=-35
     misc_feature    2313..2329
                     /label=lac O1
     promoter        2330..2335
                     /label=-10
     misc_feature    2288..2341
                     /label=BBa_R0011
     promoter        2288..2341
                     /label=BBa_R0011
     misc_feature    970..2342
                     /label=BBa_Q01121
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaac ctactagatg tccagattag ataaaagtaa agtgattaac agcgcattag
      121 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
      181 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
      241 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
      301 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
      361 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
      421 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
      481 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
      541 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
      601 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
      661 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg
      721 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca
      781 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      841 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      901 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
      961 ctactagagt cacacaggaa acctactaga tggtgaatgt gaaaccagta acgttatacg
     1021 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     1081 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     1141 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     1201 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     1261 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     1321 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     1381 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     1441 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     1501 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     1561 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     1621 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     1681 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     1741 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     1801 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     1861 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     1921 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     1981 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2041 agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg
     2101 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca
     2161 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2221 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     2281 actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag atactgagca
     2341 ca
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