LOCUS       BBa_I13657              2340 bp    DNA     linear       22-Dec-2024
DEFINITION  Cascaded Tet (elowitz) and Lac (medium) QPIs with constitutive 
            promoter as input
ACCESSION   BBa_I13657
VERSION     BBa_I13657.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             79..695
                     /label=tetR
     misc_feature    696..729
                     /label=SsrA
     misc_feature    736..760
                     /label=Help:Barcodes
     CDS             76..735
                     /label=BBa_C0040
     stem_loop       780..823
                     /label=stem_loop
     misc_feature    769..848
                     /label=BBa_B0010
     terminator      769..848
                     /label=BBa_B0010
     stem_loop       864..883
                     /label=T7 TE
     misc_feature    890..890
                     /label=stop
     polyA_site      884..897
                     /label=polya
     misc_feature    857..897
                     /label=BBa_B0012
     terminator      857..897
                     /label=BBa_B0012
     misc_feature    906..924
                     /label=TetR 1
     misc_feature    906..959
                     /label=BBa_R0040
     promoter        925..930
                     /label=-35
     misc_feature    931..949
                     /label=TetR 2
     promoter        948..953
                     /label=-10
     promoter        906..959
                     /label=BBa_R0040
     misc_feature    58..959
                     /label=BBa_Q04400
     misc_feature    974..977
                     /label=conserved
     RBS             968..981
                     /label=BBa_B0031
     misc_feature    2077..2115
                     /label=LVA
     CDS             988..2115
                     /label=lacI-LVA
     misc_feature    988..2115
                     /label=BBa_C0012
     misc_feature    2116..2140
                     /label=Help:Barcodes
     CDS             988..2115
                     /label=BBa_C0012
     stem_loop       2160..2203
                     /label=stem_loop
     misc_feature    2149..2228
                     /label=BBa_B0010
     terminator      2149..2228
                     /label=BBa_B0010
     stem_loop       2244..2263
                     /label=T7 TE
     misc_feature    2270..2270
                     /label=stop
     polyA_site      2264..2277
                     /label=polya
     misc_feature    2237..2277
                     /label=BBa_B0012
     terminator      2237..2277
                     /label=BBa_B0012
     terminator      2149..2277
                     /label=BBa_B0015
     misc_feature    2288..2304
                     /label=lac O1
     promoter        2305..2310
                     /label=-35
     misc_feature    2311..2327
                     /label=lac O1
     promoter        2328..2333
                     /label=-10
     misc_feature    2286..2339
                     /label=BBa_R0011
     promoter        2286..2339
                     /label=BBa_R0011
     misc_feature    968..2340
                     /label=BBa_Q01121
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
      121 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      181 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      241 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      301 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      361 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      421 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      481 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      541 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      601 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      661 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
      721 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa
      781 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      841 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      901 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact
      961 actagagtca cacaggaaac ctactagatg gtgaatgtga aaccagtaac gttatacgat
     1021 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
     1081 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
     1141 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
     1201 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
     1261 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
     1321 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
     1381 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
     1441 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
     1501 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
     1561 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
     1621 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
     1681 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     1741 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
     1801 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     1861 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     1921 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     1981 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2041 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct
     2101 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa
     2161 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2221 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     2281 tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca
//