LOCUS       BBa_I13664              2342 bp    DNA     linear       25-Nov-2024
DEFINITION  Cascaded Lac (medium) and Tet (medium) QPIs with constitutive 
            promoter as input
ACCESSION   BBa_I13664
VERSION     BBa_I13664.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    64..67
                     /label=conserved
     RBS             58..71
                     /label=BBa_B0031
     misc_feature    1167..1205
                     /label=LVA
     CDS             78..1205
                     /label=lacI-LVA
     misc_feature    78..1205
                     /label=BBa_C0012
     misc_feature    1206..1230
                     /label=Help:Barcodes
     CDS             78..1205
                     /label=BBa_C0012
     stem_loop       1250..1293
                     /label=stem_loop
     misc_feature    1239..1318
                     /label=BBa_B0010
     terminator      1239..1318
                     /label=BBa_B0010
     stem_loop       1334..1353
                     /label=T7 TE
     misc_feature    1360..1360
                     /label=stop
     polyA_site      1354..1367
                     /label=polya
     misc_feature    1327..1367
                     /label=BBa_B0012
     terminator      1327..1367
                     /label=BBa_B0012
     terminator      1239..1367
                     /label=BBa_B0015
     misc_feature    1378..1394
                     /label=lac O1
     promoter        1395..1400
                     /label=-35
     misc_feature    1401..1417
                     /label=lac O1
     promoter        1418..1423
                     /label=-10
     misc_feature    1376..1429
                     /label=BBa_R0011
     promoter        1376..1429
                     /label=BBa_R0011
     misc_feature    58..1430
                     /label=BBa_Q01121
     misc_feature    1445..1448
                     /label=conserved
     RBS             1439..1452
                     /label=BBa_B0031
     CDS             1462..2078
                     /label=tetR
     misc_feature    2079..2112
                     /label=SsrA
     misc_feature    2119..2143
                     /label=Help:Barcodes
     CDS             1459..2118
                     /label=BBa_C0040
     stem_loop       2163..2206
                     /label=stem_loop
     misc_feature    2152..2231
                     /label=BBa_B0010
     terminator      2152..2231
                     /label=BBa_B0010
     stem_loop       2247..2266
                     /label=T7 TE
     misc_feature    2273..2273
                     /label=stop
     polyA_site      2267..2280
                     /label=polya
     misc_feature    2240..2280
                     /label=BBa_B0012
     terminator      2240..2280
                     /label=BBa_B0012
     misc_feature    2289..2307
                     /label=TetR 1
     misc_feature    2289..2342
                     /label=BBa_R0040
     promoter        2308..2313
                     /label=-35
     misc_feature    2314..2332
                     /label=TetR 2
     promoter        2331..2336
                     /label=-10
     promoter        2289..2342
                     /label=BBa_R0040
     misc_feature    1439..2342
                     /label=BBa_Q01400
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaac ctactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
      121 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
      181 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
      241 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
      301 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
      361 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
      421 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
      481 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
      541 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
      601 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
      661 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
      721 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
      781 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
      841 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
      901 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
      961 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     1021 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     1081 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1141 aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt
     1201 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
     1261 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1321 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaattg
     1381 tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca tactagagtc
     1441 acacaggaaa cctactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
     1501 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
     1561 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
     1621 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
     1681 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
     1741 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
     1801 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
     1861 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
     1921 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
     1981 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
     2041 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
     2101 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
     2161 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2221 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2281 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     2341 ac
//