LOCUS       BBa_I13665              2340 bp    DNA     linear       18-May-2024
DEFINITION  Cascaded Lac (medium) and Tet (elowitz) QPIs with constitutive 
            promoter as input
ACCESSION   BBa_I13665
VERSION     BBa_I13665.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    64..67
                     /label=conserved
     RBS             58..71
                     /label=BBa_B0031
     misc_feature    1167..1205
                     /label=LVA
     CDS             78..1205
                     /label=lacI-LVA
     misc_feature    78..1205
                     /label=BBa_C0012
     misc_feature    1206..1230
                     /label=Help:Barcodes
     CDS             78..1205
                     /label=BBa_C0012
     stem_loop       1250..1293
                     /label=stem_loop
     misc_feature    1239..1318
                     /label=BBa_B0010
     terminator      1239..1318
                     /label=BBa_B0010
     stem_loop       1334..1353
                     /label=T7 TE
     misc_feature    1360..1360
                     /label=stop
     polyA_site      1354..1367
                     /label=polya
     misc_feature    1327..1367
                     /label=BBa_B0012
     terminator      1327..1367
                     /label=BBa_B0012
     terminator      1239..1367
                     /label=BBa_B0015
     misc_feature    1378..1394
                     /label=lac O1
     promoter        1395..1400
                     /label=-35
     misc_feature    1401..1417
                     /label=lac O1
     promoter        1418..1423
                     /label=-10
     misc_feature    1376..1429
                     /label=BBa_R0011
     promoter        1376..1429
                     /label=BBa_R0011
     misc_feature    58..1430
                     /label=BBa_Q01121
     misc_feature    1443..1446
                     /label=conserved
     RBS             1439..1450
                     /label=BBa_B0034
     CDS             1460..2076
                     /label=tetR
     misc_feature    2077..2110
                     /label=SsrA
     misc_feature    2117..2141
                     /label=Help:Barcodes
     CDS             1457..2116
                     /label=BBa_C0040
     stem_loop       2161..2204
                     /label=stem_loop
     misc_feature    2150..2229
                     /label=BBa_B0010
     terminator      2150..2229
                     /label=BBa_B0010
     stem_loop       2245..2264
                     /label=T7 TE
     misc_feature    2271..2271
                     /label=stop
     polyA_site      2265..2278
                     /label=polya
     misc_feature    2238..2278
                     /label=BBa_B0012
     terminator      2238..2278
                     /label=BBa_B0012
     misc_feature    2287..2305
                     /label=TetR 1
     misc_feature    2287..2340
                     /label=BBa_R0040
     promoter        2306..2311
                     /label=-35
     misc_feature    2312..2330
                     /label=TetR 2
     promoter        2329..2334
                     /label=-10
     promoter        2287..2340
                     /label=BBa_R0040
     misc_feature    1439..2340
                     /label=BBa_Q04400
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaac ctactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
      121 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
      181 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
      241 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
      301 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
      361 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
      421 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
      481 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
      541 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
      601 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
      661 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
      721 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
      781 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
      841 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
      901 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
      961 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     1021 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     1081 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1141 aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt
     1201 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
     1261 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1321 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaattg
     1381 tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca tactagagaa
     1441 agaggagaaa tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga
     1501 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg
     1561 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt
     1621 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg
     1681 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga
     1741 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa
     1801 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag
     1861 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa
     1921 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga
     1981 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg
     2041 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc
     2101 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa
     2161 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2221 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     2281 ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
//