LOCUS       BBa_I13667              2340 bp    DNA     linear       18-May-2024
DEFINITION  Cascaded Lac (elowitz) and Tet (medium) QPIs with constitutive 
            promoter as input
ACCESSION   BBa_I13667
VERSION     BBa_I13667.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    1165..1203
                     /label=LVA
     CDS             76..1203
                     /label=lacI-LVA
     misc_feature    76..1203
                     /label=BBa_C0012
     misc_feature    1204..1228
                     /label=Help:Barcodes
     CDS             76..1203
                     /label=BBa_C0012
     stem_loop       1248..1291
                     /label=stem_loop
     misc_feature    1237..1316
                     /label=BBa_B0010
     terminator      1237..1316
                     /label=BBa_B0010
     stem_loop       1332..1351
                     /label=T7 TE
     misc_feature    1358..1358
                     /label=stop
     polyA_site      1352..1365
                     /label=polya
     misc_feature    1325..1365
                     /label=BBa_B0012
     terminator      1325..1365
                     /label=BBa_B0012
     terminator      1237..1365
                     /label=BBa_B0015
     misc_feature    1376..1392
                     /label=lac O1
     promoter        1393..1398
                     /label=-35
     misc_feature    1399..1415
                     /label=lac O1
     promoter        1416..1421
                     /label=-10
     misc_feature    1374..1427
                     /label=BBa_R0011
     promoter        1374..1427
                     /label=BBa_R0011
     misc_feature    58..1428
                     /label=BBa_Q04121
     misc_feature    1443..1446
                     /label=conserved
     RBS             1437..1450
                     /label=BBa_B0031
     CDS             1460..2076
                     /label=tetR
     misc_feature    2077..2110
                     /label=SsrA
     misc_feature    2117..2141
                     /label=Help:Barcodes
     CDS             1457..2116
                     /label=BBa_C0040
     stem_loop       2161..2204
                     /label=stem_loop
     misc_feature    2150..2229
                     /label=BBa_B0010
     terminator      2150..2229
                     /label=BBa_B0010
     stem_loop       2245..2264
                     /label=T7 TE
     misc_feature    2271..2271
                     /label=stop
     polyA_site      2265..2278
                     /label=polya
     misc_feature    2238..2278
                     /label=BBa_B0012
     terminator      2238..2278
                     /label=BBa_B0012
     misc_feature    2287..2305
                     /label=TetR 1
     misc_feature    2287..2340
                     /label=BBa_R0040
     promoter        2306..2311
                     /label=-35
     misc_feature    2312..2330
                     /label=TetR 2
     promoter        2329..2334
                     /label=-10
     promoter        2287..2340
                     /label=BBa_R0040
     misc_feature    1437..2340
                     /label=BBa_Q01400
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
      901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
      961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1141 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa
     1201 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1321 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg
     1381 agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata ctagagtcac
     1441 acaggaaacc tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga
     1501 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg
     1561 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt
     1621 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg
     1681 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga
     1741 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa
     1801 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag
     1861 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa
     1921 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga
     1981 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg
     2041 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc
     2101 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa
     2161 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2221 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     2281 ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
//