LOCUS       BBa_I13710               610 bp    DNA     linear       26-Nov-2024
DEFINITION  CheY behind a tet promoter
ACCESSION   BBa_I13710
VERSION     BBa_I13710.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..473
                     /label=cheY
     CDS             81..473
                     /label=BBa_C0020
     stem_loop       493..536
                     /label=stem_loop
     misc_feature    482..561
                     /label=BBa_B0010
     terminator      482..561
                     /label=BBa_B0010
     stem_loop       577..596
                     /label=T7 TE
     misc_feature    603..603
                     /label=stop
     polyA_site      597..610
                     /label=polya
     misc_feature    570..610
                     /label=BBa_B0012
     terminator      570..610
                     /label=BBa_B0012
     terminator      482..610
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcggata aagaacttaa atttttggtt gtggatgact
      121 tttccaccat gcgacgcata gtgcgtaacc tgctgaaaga gctgggattc aataatgttg
      181 aggaagcgga agatggcgtc gacgctctca ataagttgca ggcaggcggt tatggatttg
      241 ttatctccga ctggaacatg cccaatatgg atggcctgga attgctgaaa acaattcgtg
      301 cggatggcgc gatgtcggca ttgccagtgt taatggtgac tgcggaagcg aagaaagaga
      361 acatcattgc tgcggcgcaa gcgggggcca gtggctatgt ggtgaagcca tttaccgccg
      421 cgacgctgga ggaaaaactc aacaaaatct ttgagaaact gggcatgtaa taatactaga
      481 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      541 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      601 tgcgtttata
//