LOCUS       BBa_I13711              1295 bp    DNA     linear       25-Nov-2024
DEFINITION  Tet promoter, Elowitz RBS, CheB
ACCESSION   BBa_I13711
VERSION     BBa_I13711.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1133
                     /label=CheB
     misc_feature    1134..1158
                     /label=Help:Barcodes
     CDS             81..1158
                     /label=BBa_C0024
     stem_loop       1178..1221
                     /label=stem_loop
     misc_feature    1167..1246
                     /label=BBa_B0010
     terminator      1167..1246
                     /label=BBa_B0010
     stem_loop       1262..1281
                     /label=T7 TE
     misc_feature    1288..1288
                     /label=stop
     polyA_site      1282..1295
                     /label=polya
     misc_feature    1255..1295
                     /label=BBa_B0012
     terminator      1255..1295
                     /label=BBa_B0012
     terminator      1167..1295
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagcaaaa tcagggtgtt atctgtcgat gattcggcac
      121 tgatgcgcca gatcatgaca gaaatcatca acagccatag cgacatggaa atggtggcga
      181 ccgcgcctga tccgctggtc gcgcgtgact tgattaagaa attcaatccc gatgtgctga
      241 cgctggatgt tgaaatgccg cggatggacg gactggattt cctcgaaaaa ttaatgcgtt
      301 tgcgtccaat gcccgttgtg atggtttctt ccctgaccgg caaagggtca gaagtcacgc
      361 tgcgcgcgct ggagctgggg gcgatagatt ttgtcaccaa accgcaactg ggtattcgcg
      421 aaggtatgct ggcgtataac gaaatgattg ctgaaaaggt gcgtacggca gcaaaggcga
      481 gccttgcagc acataagcca ttgtcggcac cgacaacgct gaaggcgggg ccgttgttga
      541 gttctgaaaa actgattgcg attggtgctt caacgggtgg aactgaggca attcgtcacg
      601 tactgcaacc gttgccgctt tccagcccgg cactgttaat tacccagcat atgccgcccg
      661 gtttcacccg ctcttttgcc gacagactta ataagctttg ccagatcggg gttaaagaag
      721 ccgaagacgg agaacgtgtc ttgccggggc atgcctatat tgcgccgggc gatcggcata
      781 tggagctgtc gcgtagtggc gcaaattacc aaatcaaaat tcacgatggc ccggcggtta
      841 accgtcatcg gccttcggta gatgtgttgt tccattctgt cgccaaacag gcggggcgta
      901 atgcggttgg ggtgatcctg accggtatgg gcaacgacgg cgcggcggga atgttggcga
      961 tgcgtcaggc gggggcatgg acccttgcgc aaaacgaagc aagttgcgtg gtgttcggca
     1021 tgccgcgcga ggccatcaat atgggtggtg tctgcgaagt ggtcgatctt agccaggtaa
     1081 gccagcaaat gttggcaaaa attagtgccg gacaggcgat acgtatttaa taacactgat
     1141 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1201 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1261 tggctcacct tcgggtgggc ctttctgcgt ttata
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