LOCUS       BBa_I13712              1106 bp    DNA     linear       22-Dec-2024
DEFINITION  CheR behind a tet promoter and Elowitz RBS
ACCESSION   BBa_I13712
VERSION     BBa_I13712.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..944
                     /label=CheB
     misc_feature    945..969
                     /label=Help:Barcodes
     CDS             81..944
                     /label=BBa_C0028
     stem_loop       989..1032
                     /label=stem_loop
     misc_feature    978..1057
                     /label=BBa_B0010
     terminator      978..1057
                     /label=BBa_B0010
     stem_loop       1073..1092
                     /label=T7 TE
     misc_feature    1099..1099
                     /label=stop
     polyA_site      1093..1106
                     /label=polya
     misc_feature    1066..1106
                     /label=BBa_B0012
     terminator      1066..1106
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgacttcat ctctgccctg tgggcaaacg tctttattgt
      121 tacagatgac cgagcgcctg gcgctttccg acgcgcattt tcggcggata agtcaattga
      181 tctatcaacg agccgggatc gttctggctg accataaacg cgacatggtt tacaaccgac
      241 tggttcgtcg tttgcgttcg ctgggactga cggatttcgg tcattatctg aacttgctgg
      301 aatctaatca gcacagcggt gagtggcagg cgtttatcaa ttcgctgacc acgaatctga
      361 cggcattttt ccgtgaggca catcatttcc ctctgctcgc ggatcacgca cgtcgccgtt
      421 ctggcgagta tcgcgtatgg agcgcggcgg cttcgaccgg cgaagagccg tacagcattg
      481 cgatgacgct ggctgacaca ttgggcaccg cgcccggacg ctggaaagtg tttgccagtg
      541 atatcgacac cgaagtgctg gaaaaagcca gaagcggtat ctatcgccat gaagagttga
      601 aaaacctgac gccgcagcaa ctgcaacggt atttcatgcg agggacgggg ccgcatgaag
      661 ggctggtacg cgtgcgtcag gagctggcga actatgttga ttttgccccg ctgaatctac
      721 tggcgaaaca gtacaccgtg ccggggccgt ttgatgcgat cttctgtcgt aacgtcatga
      781 tctacttcga tcaaactacc cagcaggaga ttttgcgccg ctttgttccg ctccttaaac
      841 ccgacggatt gctgtttgcg ggtcactctg aaaactttag ccaccttgag cgccgcttca
      901 cgctgcgtgg tcagacggtg tatgcgctaa gtaaggatta ataacgctga tagtgctagt
      961 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1021 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1081 ttcgggtggg cctttctgcg tttata
//