LOCUS       BBa_I13721              1296 bp    DNA     linear       22-Dec-2024
DEFINITION  CheB under Lac control with Elowitz RBS
ACCESSION   BBa_I13721
VERSION     BBa_I13721.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1134
                     /label=CheB
     misc_feature    1135..1159
                     /label=Help:Barcodes
     CDS             82..1159
                     /label=BBa_C0024
     stem_loop       1179..1222
                     /label=stem_loop
     misc_feature    1168..1247
                     /label=BBa_B0010
     terminator      1168..1247
                     /label=BBa_B0010
     stem_loop       1263..1282
                     /label=T7 TE
     misc_feature    1289..1289
                     /label=stop
     polyA_site      1283..1296
                     /label=polya
     misc_feature    1256..1296
                     /label=BBa_B0012
     terminator      1256..1296
                     /label=BBa_B0012
     terminator      1168..1296
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaaa atcagggtgt tatctgtcga tgattcggca
      121 ctgatgcgcc agatcatgac agaaatcatc aacagccata gcgacatgga aatggtggcg
      181 accgcgcctg atccgctggt cgcgcgtgac ttgattaaga aattcaatcc cgatgtgctg
      241 acgctggatg ttgaaatgcc gcggatggac ggactggatt tcctcgaaaa attaatgcgt
      301 ttgcgtccaa tgcccgttgt gatggtttct tccctgaccg gcaaagggtc agaagtcacg
      361 ctgcgcgcgc tggagctggg ggcgatagat tttgtcacca aaccgcaact gggtattcgc
      421 gaaggtatgc tggcgtataa cgaaatgatt gctgaaaagg tgcgtacggc agcaaaggcg
      481 agccttgcag cacataagcc attgtcggca ccgacaacgc tgaaggcggg gccgttgttg
      541 agttctgaaa aactgattgc gattggtgct tcaacgggtg gaactgaggc aattcgtcac
      601 gtactgcaac cgttgccgct ttccagcccg gcactgttaa ttacccagca tatgccgccc
      661 ggtttcaccc gctcttttgc cgacagactt aataagcttt gccagatcgg ggttaaagaa
      721 gccgaagacg gagaacgtgt cttgccgggg catgcctata ttgcgccggg cgatcggcat
      781 atggagctgt cgcgtagtgg cgcaaattac caaatcaaaa ttcacgatgg cccggcggtt
      841 aaccgtcatc ggccttcggt agatgtgttg ttccattctg tcgccaaaca ggcggggcgt
      901 aatgcggttg gggtgatcct gaccggtatg ggcaacgacg gcgcggcggg aatgttggcg
      961 atgcgtcagg cgggggcatg gacccttgcg caaaacgaag caagttgcgt ggtgttcggc
     1021 atgccgcgcg aggccatcaa tatgggtggt gtctgcgaag tggtcgatct tagccaggta
     1081 agccagcaaa tgttggcaaa aattagtgcc ggacaggcga tacgtattta ataacactga
     1141 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1201 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1261 ctggctcacc ttcgggtggg cctttctgcg tttata
//