BBa_I13730
type: ComponentDefinition
persistent identity: BBa I13730
display id: BBa_I13730
version: 1
was generated by: iGEM to SBOL conversion
description: Lac CheB, Tet CheR
created: 2004-08-12T11:00:00Z
modified: 2015-08-31T04:07:36Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13721
component: BBa_I13712
sequence annotation: BBa_I13721
sequence annotation: BBa_I13712
sequence: https://synbiohub.org/public/igem/BBa_I13730_sequence/1

BBa_I13721
type: Component
persistent identity: component2229672
display id: component2229672
version: 1
top level: BBa_I13730
definition: BBa_I13721
access: public

BBa_I13712
type: Component
persistent identity: component2229688
display id: component2229688
version: 1
top level: BBa_I13730
definition: BBa_I13712
access: public

BBa_I13721
type: SequenceAnnotation
persistent identity: annotation2229672
display id: annotation2229672
version: 1
top level: BBa_I13730
location: https://synbiohub.org/public/igem/BBa_I13730/annotation2229672/range2229672/1
component: BBa_I13721

https://synbiohub.org/public/igem/BBa_I13730/annotation2229672/range2229672/1
type: Range
persistent identity: range2229672
display id: range2229672
version: 1
top level: BBa_I13730
direction: direction/reverse
start: 1
end: 1296
orientation: inline

BBa_I13712
type: SequenceAnnotation
persistent identity: annotation2229688
display id: annotation2229688
version: 1
top level: BBa_I13730
location: https://synbiohub.org/public/igem/BBa_I13730/annotation2229688/range2229688/1
component: BBa_I13712

https://synbiohub.org/public/igem/BBa_I13730/annotation2229688/range2229688/1
type: Range
persistent identity: range2229688
display id: range2229688
version: 1
top level: BBa_I13730
direction: direction/reverse
start: 1305
end: 2410
orientation: inline

BBa_I13712
type: ComponentDefinition
persistent identity: BBa I13712
display id: BBa_I13712
version: 1
was generated by: iGEM to SBOL conversion
description: CheR behind a tet promoter and Elowitz RBS
created: 2004-08-12T11:00:00Z
modified: 2015-08-31T04:07:36Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_C0028
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0028
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13712_sequence/1

BBa_B0012
type: Component
persistent identity: component1045384
display id: component1045384
version: 1
top level: BBa_I13712
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1045374
display id: component1045374
version: 1
top level: BBa_I13712
definition: BBa_B0010
access: public

BBa_R0040
type: Component
persistent identity: component1045356
display id: component1045356
version: 1
top level: BBa_I13712
definition: p(tetR)
access: public

BBa_C0028
type: Component
persistent identity: component1045368
display id: component1045368
version: 1
top level: BBa_I13712
definition: cher
access: public

BBa_B0034
type: Component
persistent identity: component1045364
display id: component1045364
version: 1
top level: BBa_I13712
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1045356
display id: annotation1045356
version: 1
top level: BBa_I13712
location: https://synbiohub.org/public/igem/BBa_I13712/annotation1045356/range1045356/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13712/annotation1045356/range1045356/1
type: Range
persistent identity: range1045356
display id: range1045356
version: 1
top level: BBa_I13712
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1045364
display id: annotation1045364
version: 1
top level: BBa_I13712
location: https://synbiohub.org/public/igem/BBa_I13712/annotation1045364/range1045364/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13712/annotation1045364/range1045364/1
type: Range
persistent identity: range1045364
display id: range1045364
version: 1
top level: BBa_I13712
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_C0028
type: SequenceAnnotation
persistent identity: annotation1045368
display id: annotation1045368
version: 1
top level: BBa_I13712
location: https://synbiohub.org/public/igem/BBa_I13712/annotation1045368/range1045368/1
component: BBa_C0028

https://synbiohub.org/public/igem/BBa_I13712/annotation1045368/range1045368/1
type: Range
persistent identity: range1045368
display id: range1045368
version: 1
top level: BBa_I13712
direction: direction/forward
start: 81
end: 944
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1045374
display id: annotation1045374
version: 1
top level: BBa_I13712
location: https://synbiohub.org/public/igem/BBa_I13712/annotation1045374/range1045374/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13712/annotation1045374/range1045374/1
type: Range
persistent identity: range1045374
display id: range1045374
version: 1
top level: BBa_I13712
direction: direction/forward
start: 978
end: 1057
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1045384
display id: annotation1045384
version: 1
top level: BBa_I13712
location: https://synbiohub.org/public/igem/BBa_I13712/annotation1045384/range1045384/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13712/annotation1045384/range1045384/1
type: Range
persistent identity: range1045384
display id: range1045384
version: 1
top level: BBa_I13712
direction: direction/forward
start: 1066
end: 1106
orientation: inline

BBa_I13721
type: ComponentDefinition
persistent identity: BBa I13721
display id: BBa_I13721
version: 1
was generated by: iGEM to SBOL conversion
description: CheB under Lac control with Elowitz RBS
created: 2004-08-12T11:00:00Z
modified: 2015-08-31T04:07:36Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_R0011
component: BBa_B0015
component: BBa_C0024
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0024
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I13721_sequence/1

BBa_B0034
type: Component
persistent identity: component2221641
display id: component2221641
version: 1
top level: BBa_I13721
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2221635
display id: component2221635
version: 1
top level: BBa_I13721
definition: lacI+pL
access: public

BBa_B0015
type: Component
persistent identity: component2221651
display id: component2221651
version: 1
top level: BBa_I13721
definition: BBa_B0015
access: public

BBa_C0024
type: Component
persistent identity: component2221644
display id: component2221644
version: 1
top level: BBa_I13721
definition: cheB
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221635
display id: annotation2221635
version: 1
top level: BBa_I13721
location: https://synbiohub.org/public/igem/BBa_I13721/annotation2221635/range2221635/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13721/annotation2221635/range2221635/1
type: Range
persistent identity: range2221635
display id: range2221635
version: 1
top level: BBa_I13721
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221641
display id: annotation2221641
version: 1
top level: BBa_I13721
location: https://synbiohub.org/public/igem/BBa_I13721/annotation2221641/range2221641/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13721/annotation2221641/range2221641/1
type: Range
persistent identity: range2221641
display id: range2221641
version: 1
top level: BBa_I13721
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_C0024
type: SequenceAnnotation
persistent identity: annotation2221644
display id: annotation2221644
version: 1
top level: BBa_I13721
location: https://synbiohub.org/public/igem/BBa_I13721/annotation2221644/range2221644/1
component: BBa_C0024

https://synbiohub.org/public/igem/BBa_I13721/annotation2221644/range2221644/1
type: Range
persistent identity: range2221644
display id: range2221644
version: 1
top level: BBa_I13721
direction: direction/reverse
start: 82
end: 1159
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221651
display id: annotation2221651
version: 1
top level: BBa_I13721
location: https://synbiohub.org/public/igem/BBa_I13721/annotation2221651/range2221651/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13721/annotation2221651/range2221651/1
type: Range
persistent identity: range2221651
display id: range2221651
version: 1
top level: BBa_I13721
direction: direction/reverse
start: 1168
end: 1296
orientation: inline

cheB
type: ComponentDefinition
persistent identity: BBa C0024
display id: BBa_C0024
version: 1
was generated by: iGEM to SBOL conversion
description: CheB chemotaxis coding sequence (protein glutamate methylesterase)
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: NCBI - E. Coli strain K12 substrain MG1655
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: CheB is part of the chemotaxis pathway in E. coli.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Checked (DE, VC, KN). Part ready for synthesis. 8/3/4
star: true
creator: MIT SBC 2004 (Ken)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CheB
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0024_sequence/1

CheB
type: SequenceAnnotation
persistent identity: annotation1891614
display id: annotation1891614
version: 1
top level: cheB
location: https://synbiohub.org/public/igem/BBa_C0024/annotation1891614/range1891614/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0024/annotation1891614/range1891614/1
type: Range
persistent identity: range1891614
display id: range1891614
version: 1
top level: cheB
direction: direction/forward
start: 1
end: 1053
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214017
display id: annotation2214017
version: 1
top level: cheB
location: https://synbiohub.org/public/igem/BBa_C0024/annotation2214017/range2214017/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0024/annotation2214017/range2214017/1
type: Range
persistent identity: range2214017
display id: range2214017
version: 1
top level: cheB
direction: direction/forward
start: 1054
end: 1078
orientation: inline

cher
type: ComponentDefinition
persistent identity: BBa C0028
display id: BBa_C0028
version: 1
was generated by: iGEM to SBOL conversion
description: CheR chemotaxis coding region
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: NCBI - E. Coli K12 substrain: MG1655
owned by: james
owned by: myers
mutable description: CheR is part of the chemotaxis pathway in E. Coli. (Was part # I13702)
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Checked (DE, VC, KN). Part ready for synthesis (8/3/4) sequence data at http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946396
star: false
creator: MIT SBC 2004 (Ken)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CheB
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0028_sequence/1

CheB
type: SequenceAnnotation
persistent identity: annotation1891584
display id: annotation1891584
version: 1
top level: cher
location: https://synbiohub.org/public/igem/BBa_C0028/annotation1891584/range1891584/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0028/annotation1891584/range1891584/1
type: Range
persistent identity: range1891584
display id: range1891584
version: 1
top level: cher
direction: direction/forward
start: 1
end: 864
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214018
display id: annotation2214018
version: 1
top level: cher
location: https://synbiohub.org/public/igem/BBa_C0028/annotation2214018/range2214018/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0028/annotation2214018/range2214018/1
type: Range
persistent identity: range2214018
display id: range2214018
version: 1
top level: cher
direction: direction/forward
start: 865
end: 889
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_I13730_sequence/1
type: Sequence
persistent identity: BBa I13730 sequence
display id: BBa_I13730_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcaaaatcagggtgttatctgtcgatgattcggcactgatgcgccagatcatgacagaaatcatcaacagccatagcgacatggaaatggtggcgaccgcgcctgatccgctggtcgcgcgtgacttgattaagaaattcaatcccgatgtgctgacgctggatgttgaaatgccgcggatggacggactggatttcctcgaaaaattaatgcgtttgcgtccaatgcccgttgtgatggtttcttccctgaccggcaaagggtcagaagtcacgctgcgcgcgctggagctgggggcgatagattttgtcaccaaaccgcaactgggtattcgcgaaggtatgctggcgtataacgaaatgattgctgaaaaggtgcgtacggcagcaaaggcgagccttgcagcacataagccattgtcggcaccgacaacgctgaaggcggggccgttgttgagttctgaaaaactgattgcgattggtgcttcaacgggtggaactgaggcaattcgtcacgtactgcaaccgttgccgctttccagcccggcactgttaattacccagcatatgccgcccggtttcacccgctcttttgccgacagacttaataagctttgccagatcggggttaaagaagccgaagacggagaacgtgtcttgccggggcatgcctatattgcgccgggcgatcggcatatggagctgtcgcgtagtggcgcaaattaccaaatcaaaattcacgatggcccggcggttaaccgtcatcggccttcggtagatgtgttgttccattctgtcgccaaacaggcggggcgtaatgcggttggggtgatcctgaccggtatgggcaacgacggcgcggcgggaatgttggcgatgcgtcaggcgggggcatggacccttgcgcaaaacgaagcaagttgcgtggtgttcggcatgccgcgcgaggccatcaatatgggtggtgtctgcgaagtggtcgatcttagccaggtaagccagcaaatgttggcaaaaattagtgccggacaggcgatacgtatttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgacttcatctctgccctgtgggcaaacgtctttattgttacagatgaccgagcgcctggcgctttccgacgcgcattttcggcggataagtcaattgatctatcaacgagccgggatcgttctggctgaccataaacgcgacatggtttacaaccgactggttcgtcgtttgcgttcgctgggactgacggatttcggtcattatctgaacttgctggaatctaatcagcacagcggtgagtggcaggcgtttatcaattcgctgaccacgaatctgacggcatttttccgtgaggcacatcatttccctctgctcgcggatcacgcacgtcgccgttctggcgagtatcgcgtatggagcgcggcggcttcgaccggcgaagagccgtacagcattgcgatgacgctggctgacacattgggcaccgcgcccggacgctggaaagtgtttgccagtgatatcgacaccgaagtgctggaaaaagccagaagcggtatctatcgccatgaagagttgaaaaacctgacgccgcagcaactgcaacggtatttcatgcgagggacggggccgcatgaagggctggtacgcgtgcgtcaggagctggcgaactatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13712_sequence/1
type: Sequence
persistent identity: BBa I13712 sequence
display id: BBa_I13712_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgacttcatctctgccctgtgggcaaacgtctttattgttacagatgaccgagcgcctggcgctttccgacgcgcattttcggcggataagtcaattgatctatcaacgagccgggatcgttctggctgaccataaacgcgacatggtttacaaccgactggttcgtcgtttgcgttcgctgggactgacggatttcggtcattatctgaacttgctggaatctaatcagcacagcggtgagtggcaggcgtttatcaattcgctgaccacgaatctgacggcatttttccgtgaggcacatcatttccctctgctcgcggatcacgcacgtcgccgttctggcgagtatcgcgtatggagcgcggcggcttcgaccggcgaagagccgtacagcattgcgatgacgctggctgacacattgggcaccgcgcccggacgctggaaagtgtttgccagtgatatcgacaccgaagtgctggaaaaagccagaagcggtatctatcgccatgaagagttgaaaaacctgacgccgcagcaactgcaacggtatttcatgcgagggacggggccgcatgaagggctggtacgcgtgcgtcaggagctggcgaactatgttgattttgccccgctgaatctactggcgaaacagtacaccgtgccggggccgtttgatgcgatcttctgtcgtaacgtcatgatctacttcgatcaaactacccagcaggagattttgcgccgctttgttccgctccttaaacccgacggattgctgtttgcgggtcactctgaaaactttagccaccttgagcgccgcttcacgctgcgtggtcagacggtgtatgcgctaagtaaggattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13721_sequence/1
type: Sequence
persistent identity: BBa I13721 sequence
display id: BBa_I13721_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcaaaatcagggtgttatctgtcgatgattcggcactgatgcgccagatcatgacagaaatcatcaacagccatagcgacatggaaatggtggcgaccgcgcctgatccgctggtcgcgcgtgacttgattaagaaattcaatcccgatgtgctgacgctggatgttgaaatgccgcggatggacggactggatttcctcgaaaaattaatgcgtttgcgtccaatgcccgttgtgatggtttcttccctgaccggcaaagggtcagaagtcacgctgcgcgcgctggagctgggggcgatagattttgtcaccaaaccgcaactgggtattcgcgaaggtatgctggcgtataacgaaatgattgctgaaaaggtgcgtacggcagcaaaggcgagccttgcagcacataagccattgtcggcaccgacaacgctgaaggcggggccgttgttgagttctgaaaaactgattgcgattggtgcttcaacgggtggaactgaggcaattcgtcacgtactgcaaccgttgccgctttccagcccggcactgttaattacccagcatatgccgcccggtttcacccgctcttttgccgacagacttaataagctttgccagatcggggttaaagaagccgaagacggagaacgtgtcttgccggggcatgcctatattgcgccgggcgatcggcatatggagctgtcgcgtagtggcgcaaattaccaaatcaaaattcacgatggcccggcggttaaccgtcatcggccttcggtagatgtgttgttccattctgtcgccaaacaggcggggcgtaatgcggttggggtgatcctgaccggtatgggcaacgacggcgcggcgggaatgttggcgatgcgtcaggcgggggcatggacccttgcgcaaaacgaagcaagttgcgtggtgttcggcatgccgcgcgaggccatcaatatgggtggtgtctgcgaagtggtcgatcttagccaggtaagccagcaaatgttggcaaaaattagtgccggacaggcgatacgtatttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0024_sequence/1
type: Sequence
persistent identity: BBa C0024 sequence
display id: BBa_C0024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaaaatcagggtgttatctgtcgatgattcggcactgatgcgccagatcatgacagaaatcatcaacagccatagcgacatggaaatggtggcgaccgcgcctgatccgctggtcgcgcgtgacttgattaagaaattcaatcccgatgtgctgacgctggatgttgaaatgccgcggatggacggactggatttcctcgaaaaattaatgcgtttgcgtccaatgcccgttgtgatggtttcttccctgaccggcaaagggtcagaagtcacgctgcgcgcgctggagctgggggcgatagattttgtcaccaaaccgcaactgggtattcgcgaaggtatgctggcgtataacgaaatgattgctgaaaaggtgcgtacggcagcaaaggcgagccttgcagcacataagccattgtcggcaccgacaacgctgaaggcggggccgttgttgagttctgaaaaactgattgcgattggtgcttcaacgggtggaactgaggcaattcgtcacgtactgcaaccgttgccgctttccagcccggcactgttaattacccagcatatgccgcccggtttcacccgctcttttgccgacagacttaataagctttgccagatcggggttaaagaagccgaagacggagaacgtgtcttgccggggcatgcctatattgcgccgggcgatcggcatatggagctgtcgcgtagtggcgcaaattaccaaatcaaaattcacgatggcccggcggttaaccgtcatcggccttcggtagatgtgttgttccattctgtcgccaaacaggcggggcgtaatgcggttggggtgatcctgaccggtatgggcaacgacggcgcggcgggaatgttggcgatgcgtcaggcgggggcatggacccttgcgcaaaacgaagcaagttgcgtggtgttcggcatgccgcgcgaggccatcaatatgggtggtgtctgcgaagtggtcgatcttagccaggtaagccagcaaatgttggcaaaaattagtgccggacaggcgatacgtatttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0028_sequence/1
type: Sequence
persistent identity: BBa C0028 sequence
display id: BBa_C0028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacttcatctctgccctgtgggcaaacgtctttattgttacagatgaccgagcgcctggcgctttccgacgcgcattttcggcggataagtcaattgatctatcaacgagccgggatcgttctggctgaccataaacgcgacatggtttacaaccgactggttcgtcgtttgcgttcgctgggactgacggatttcggtcattatctgaacttgctggaatctaatcagcacagcggtgagtggcaggcgtttatcaattcgctgaccacgaatctgacggcatttttccgtgaggcacatcatttccctctgctcgcggatcacgcacgtcgccgttctggcgagtatcgcgtatggagcgcggcggcttcgaccggcgaagagccgtacagcattgcgatgacgctggctgacacattgggcaccgcgcccggacgctggaaagtgtttgccagtgatatcgacaccgaagtgctggaaaaagccagaagcggtatctatcgccatgaagagttgaaaaacctgacgccgcagcaactgcaacggtatttcatgcgagggacggggccgcatgaagggctggtacgcgtgcgtcaggagctggcgaactatgttgattttgccccgctgaatctactggcgaaacagtacaccgtgccggggccgtttgatgcgatcttctgtcgtaacgtcatgatctacttcgatcaaactacccagcaggagattttgcgccgctttgttccgctccttaaacccgacggattgctgtttgcgggtcactctgaaaactttagccaccttgagcgccgcttcacgctgcgtggtcagacggtgtatgcgctaagtaaggattaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z