LOCUS       BBa_I13900              2739 bp    DNA     linear       22-Nov-2024
DEFINITION  Synchronized Brickilator Test Construct (I13201.Q04400.I0461)
ACCESSION   BBa_I13900
VERSION     BBa_I13900.1  
FEATURES             Location/Qualifiers
     promoter        1..1023
                     /label=BBa_I13201
     misc_feature    1036..1039
                     /label=conserved
     RBS             1032..1043
                     /label=BBa_B0034
     CDS             1053..1669
                     /label=tetR
     misc_feature    1670..1703
                     /label=SsrA
     misc_feature    1710..1734
                     /label=Help:Barcodes
     CDS             1050..1709
                     /label=BBa_C0040
     stem_loop       1754..1797
                     /label=stem_loop
     misc_feature    1743..1822
                     /label=BBa_B0010
     terminator      1743..1822
                     /label=BBa_B0010
     stem_loop       1838..1857
                     /label=T7 TE
     misc_feature    1864..1864
                     /label=stop
     polyA_site      1858..1871
                     /label=polya
     misc_feature    1831..1871
                     /label=BBa_B0012
     terminator      1831..1871
                     /label=BBa_B0012
     misc_feature    1880..1898
                     /label=TetR 1
     misc_feature    1880..1933
                     /label=BBa_R0040
     promoter        1899..1904
                     /label=-35
     misc_feature    1905..1923
                     /label=TetR 2
     promoter        1922..1927
                     /label=-10
     promoter        1880..1933
                     /label=BBa_R0040
     misc_feature    1946..1949
                     /label=conserved
     RBS             1942..1953
                     /label=BBa_B0034
     misc_feature    2539..2570
                     /label=LVA
     CDS             1960..2538
                     /label=luxI
     misc_feature    1960..2577
                     /label=BBa_C0061
     misc_feature    2578..2602
                     /label=Help:Barcodes
     CDS             1960..2577
                     /label=BBa_C0061
     stem_loop       2622..2665
                     /label=stem_loop
     misc_feature    2611..2690
                     /label=BBa_B0010
     terminator      2611..2690
                     /label=BBa_B0010
     stem_loop       2706..2725
                     /label=T7 TE
     misc_feature    2732..2732
                     /label=stop
     polyA_site      2726..2739
                     /label=polya
     misc_feature    2699..2739
                     /label=BBa_B0012
     terminator      2699..2739
                     /label=BBa_B0012
ORIGIN
        1 ttaattttta aagtatgggc aatcaattgc tcctgttaaa attgctttag aaatactttg
       61 gcagcggttt gttgtattga gtttcatttg cgcattggtt aaatggaaag tgacagtacg
      121 ctcactgcag cctaatattt ttgaaatatc ccaagagctt tttccttcgc atgcccacgc
      181 taaacattct ttttctcttt tggttaaatc gttgtttgat ttattatttg ctatatttat
      241 ttttcgataa ttatcaacta gagaaggaac aattaatggt atgttcatac acgcatgtaa
      301 aaataaacta tctatatagt tgtctttttc tgaatgtgca aaactaagca ttccgaagcc
      361 attgttagcc gtatgaatag ggaaactaaa cccagtgata agacctgatg ttttcgcttc
      421 tttaattaca tttggagatt ttttatttac agcattgttt tcaaatatat tccaattaat
      481 tggtgaatga ttggagttag aataatctac tataggatca tattttatta aattagcgtc
      541 atcataatat tgcctccatt ttttagggta attatctaga attgaaatat cagatttaac
      601 catagaatga ggataaatga tcgcgagtaa ataatattca caatgtacca ttttagtcat
      661 atcagataag cattgattaa tatcattatt gcttctacaa gctttaattt tattaattat
      721 tctgtatgtg tcgtcggcat ttatgttttt catacccatc tctttatcct tacctattgt
      781 ttgtcgcaag ttttgcgtgt tatatatcat taaaacggta atggattgac atcctattgt
      841 ttgtcgcaag ttttgcgtgt tatatatcat taaaacggta atagattgac atttgattct
      901 aataaattgg atttttgtca cactattata tcgcttgaaa tacaattgtt taacataagt
      961 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ttaaacgcaa
     1021 gggtactaga gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta
     1081 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg
     1141 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt
     1201 tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag
     1261 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac
     1321 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg
     1381 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat
     1441 tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc
     1501 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac
     1561 gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg
     1621 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg
     1681 acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag
     1741 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1801 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1861 ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag
     1921 agatactgag cactactaga gaaagaggag aaatactaga tgactataat gataaaaaaa
     1981 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa
     2041 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag
     2101 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga
     2161 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg
     2221 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta
     2281 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa
     2341 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca
     2401 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa
     2461 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt
     2521 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc
     2581 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt
     2641 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2701 acactggctc accttcgggt gggcctttct gcgtttata
//