LOCUS       BBa_I13902              2824 bp    DNA     linear       14-May-2024
DEFINITION  Synchronized Brickilator Test Construct (I13201.Q04510.I0461)
ACCESSION   BBa_I13902
VERSION     BBa_I13902.1  
FEATURES             Location/Qualifiers
     promoter        1..1023
                     /label=BBa_I13201
     misc_feature    1036..1039
                     /label=conserved
     RBS             1032..1043
                     /label=BBa_B0034
     CDS             1053..1760
                     /label=cI lambda
     misc_feature    1761..1793
                     /label=LVA
     misc_feature    1800..1824
                     /label=Help:Barcodes
     CDS             1050..1799
                     /label=BBa_C0051
     stem_loop       1844..1887
                     /label=stem_loop
     misc_feature    1833..1912
                     /label=BBa_B0010
     terminator      1833..1912
                     /label=BBa_B0010
     stem_loop       1928..1947
                     /label=T7 TE
     misc_feature    1954..1954
                     /label=stop
     polyA_site      1948..1961
                     /label=polya
     misc_feature    1921..1961
                     /label=BBa_B0012
     terminator      1921..1961
                     /label=BBa_B0012
     promoter        2007..2012
                     /label=-10
     promoter        1984..1989
                     /label=-35
     misc_feature    1994..2010
                     /label=OR1
     misc_feature    1970..1986
                     /label=OR2
     misc_feature    1970..2018
                     /label=BBa_R0051
     promoter        1970..2018
                     /label=BBa_R0051
     misc_feature    2031..2034
                     /label=conserved
     RBS             2027..2038
                     /label=BBa_B0034
     misc_feature    2624..2655
                     /label=LVA
     CDS             2045..2623
                     /label=luxI
     misc_feature    2045..2662
                     /label=BBa_C0061
     misc_feature    2663..2687
                     /label=Help:Barcodes
     CDS             2045..2662
                     /label=BBa_C0061
     stem_loop       2707..2750
                     /label=stem_loop
     misc_feature    2696..2775
                     /label=BBa_B0010
     terminator      2696..2775
                     /label=BBa_B0010
     stem_loop       2791..2810
                     /label=T7 TE
     misc_feature    2817..2817
                     /label=stop
     polyA_site      2811..2824
                     /label=polya
     misc_feature    2784..2824
                     /label=BBa_B0012
     terminator      2784..2824
                     /label=BBa_B0012
ORIGIN
        1 ttaattttta aagtatgggc aatcaattgc tcctgttaaa attgctttag aaatactttg
       61 gcagcggttt gttgtattga gtttcatttg cgcattggtt aaatggaaag tgacagtacg
      121 ctcactgcag cctaatattt ttgaaatatc ccaagagctt tttccttcgc atgcccacgc
      181 taaacattct ttttctcttt tggttaaatc gttgtttgat ttattatttg ctatatttat
      241 ttttcgataa ttatcaacta gagaaggaac aattaatggt atgttcatac acgcatgtaa
      301 aaataaacta tctatatagt tgtctttttc tgaatgtgca aaactaagca ttccgaagcc
      361 attgttagcc gtatgaatag ggaaactaaa cccagtgata agacctgatg ttttcgcttc
      421 tttaattaca tttggagatt ttttatttac agcattgttt tcaaatatat tccaattaat
      481 tggtgaatga ttggagttag aataatctac tataggatca tattttatta aattagcgtc
      541 atcataatat tgcctccatt ttttagggta attatctaga attgaaatat cagatttaac
      601 catagaatga ggataaatga tcgcgagtaa ataatattca caatgtacca ttttagtcat
      661 atcagataag cattgattaa tatcattatt gcttctacaa gctttaattt tattaattat
      721 tctgtatgtg tcgtcggcat ttatgttttt catacccatc tctttatcct tacctattgt
      781 ttgtcgcaag ttttgcgtgt tatatatcat taaaacggta atggattgac atcctattgt
      841 ttgtcgcaag ttttgcgtgt tatatatcat taaaacggta atagattgac atttgattct
      901 aataaattgg atttttgtca cactattata tcgcttgaaa tacaattgtt taacataagt
      961 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ttaaacgcaa
     1021 gggtactaga gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag
     1081 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg
     1141 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt
     1201 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca
     1261 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag
     1321 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc
     1381 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg
     1441 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca
     1501 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc
     1561 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta
     1621 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt
     1681 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt
     1741 ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1801 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     1861 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1921 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt aacaccgtgc
     1981 gtgttgacta ttttacctct ggcggtgata atggttgcta ctagagaaag aggagaaata
     2041 ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa
     2101 aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt
     2161 agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg
     2221 tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat
     2281 gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat
     2341 agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag
     2401 tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac
     2461 agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc
     2521 ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt
     2581 gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa
     2641 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagccagg
     2701 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2761 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2821 tata
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