LOCUS BBa_I13902 2824 bp DNA linear 25-Dec-2024 DEFINITION Synchronized Brickilator Test Construct (I13201.Q04510.I0461) ACCESSION BBa_I13902 VERSION BBa_I13902.1 FEATURES Location/Qualifiers promoter 1..1023 /label=BBa_I13201 misc_feature 1036..1039 /label=conserved RBS 1032..1043 /label=BBa_B0034 CDS 1053..1760 /label=cI lambda misc_feature 1761..1793 /label=LVA misc_feature 1800..1824 /label=Help:Barcodes CDS 1050..1799 /label=BBa_C0051 stem_loop 1844..1887 /label=stem_loop misc_feature 1833..1912 /label=BBa_B0010 terminator 1833..1912 /label=BBa_B0010 stem_loop 1928..1947 /label=T7 TE misc_feature 1954..1954 /label=stop polyA_site 1948..1961 /label=polya misc_feature 1921..1961 /label=BBa_B0012 terminator 1921..1961 /label=BBa_B0012 promoter 2007..2012 /label=-10 promoter 1984..1989 /label=-35 misc_feature 1994..2010 /label=OR1 misc_feature 1970..1986 /label=OR2 misc_feature 1970..2018 /label=BBa_R0051 promoter 1970..2018 /label=BBa_R0051 misc_feature 2031..2034 /label=conserved RBS 2027..2038 /label=BBa_B0034 misc_feature 2624..2655 /label=LVA CDS 2045..2623 /label=luxI misc_feature 2045..2662 /label=BBa_C0061 misc_feature 2663..2687 /label=Help:Barcodes CDS 2045..2662 /label=BBa_C0061 stem_loop 2707..2750 /label=stem_loop misc_feature 2696..2775 /label=BBa_B0010 terminator 2696..2775 /label=BBa_B0010 stem_loop 2791..2810 /label=T7 TE misc_feature 2817..2817 /label=stop polyA_site 2811..2824 /label=polya misc_feature 2784..2824 /label=BBa_B0012 terminator 2784..2824 /label=BBa_B0012 ORIGIN 1 ttaattttta aagtatgggc aatcaattgc tcctgttaaa attgctttag aaatactttg 61 gcagcggttt gttgtattga gtttcatttg cgcattggtt aaatggaaag tgacagtacg 121 ctcactgcag cctaatattt ttgaaatatc ccaagagctt tttccttcgc atgcccacgc 181 taaacattct ttttctcttt tggttaaatc gttgtttgat ttattatttg ctatatttat 241 ttttcgataa ttatcaacta gagaaggaac aattaatggt atgttcatac acgcatgtaa 301 aaataaacta tctatatagt tgtctttttc tgaatgtgca aaactaagca ttccgaagcc 361 attgttagcc gtatgaatag ggaaactaaa cccagtgata agacctgatg ttttcgcttc 421 tttaattaca tttggagatt ttttatttac agcattgttt tcaaatatat tccaattaat 481 tggtgaatga ttggagttag aataatctac tataggatca tattttatta aattagcgtc 541 atcataatat tgcctccatt ttttagggta attatctaga attgaaatat cagatttaac 601 catagaatga ggataaatga tcgcgagtaa ataatattca caatgtacca ttttagtcat 661 atcagataag cattgattaa tatcattatt gcttctacaa gctttaattt tattaattat 721 tctgtatgtg tcgtcggcat ttatgttttt catacccatc tctttatcct tacctattgt 781 ttgtcgcaag ttttgcgtgt tatatatcat taaaacggta atggattgac atcctattgt 841 ttgtcgcaag ttttgcgtgt tatatatcat taaaacggta atagattgac atttgattct 901 aataaattgg atttttgtca cactattata tcgcttgaaa tacaattgtt taacataagt 961 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ttaaacgcaa 1021 gggtactaga gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag 1081 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg 1141 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt 1201 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca 1261 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag 1321 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc 1381 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg 1441 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca 1501 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc 1561 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta 1621 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt 1681 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt 1741 ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac 1801 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca 1861 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1921 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt aacaccgtgc 1981 gtgttgacta ttttacctct ggcggtgata atggttgcta ctagagaaag aggagaaata 2041 ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa 2101 aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt 2161 agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg 2221 tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat 2281 gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat 2341 agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag 2401 tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac 2461 agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc 2521 ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt 2581 gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa 2641 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagccagg 2701 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2761 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2821 tata //