LOCUS BBa_I13904 3856 bp DNA linear 22-Nov-2024 DEFINITION Ring Oscillator Synchronization Device (tet) ACCESSION BBa_I13904 VERSION BBa_I13904.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 832..837 /label=stop misc_feature 793..831 /label=AAV misc_feature 76..837 /label=BBa_E0034 stem_loop 857..900 /label=stem_loop misc_feature 846..925 /label=BBa_B0010 terminator 846..925 /label=BBa_B0010 stem_loop 941..960 /label=T7 TE misc_feature 967..967 /label=stop polyA_site 961..974 /label=polya misc_feature 934..974 /label=BBa_B0012 terminator 934..974 /label=BBa_B0012 misc_feature 983..1002 /label=LuxR/HSL promoter 1097..1104 /label=-10 promoter 1071..1076 /label=-35 misc_feature 1110..1110 /label=start misc_feature 1112..1131 /label=Putative LuxR/HSL misc_feature 983..1133 /label=BBa_R0063 promoter 983..1133 /label=BBa_R0063 misc_feature 1146..1149 /label=conserved RBS 1142..1153 /label=BBa_B0034 misc_feature 1160..1161 /label=prefix misc_feature 1333..1333 /label=T misc_feature 1651..1651 /label=A CDS 1160..1909 /label=luxR misc_feature 1160..1915 /label=BBa_C0062 misc_feature 1916..1940 /label=Help:Barcodes CDS 1160..1915 /label=BBa_C0062 stem_loop 1960..2003 /label=stem_loop misc_feature 1949..2028 /label=BBa_B0010 terminator 1949..2028 /label=BBa_B0010 stem_loop 2044..2063 /label=T7 TE misc_feature 2070..2070 /label=stop polyA_site 2064..2077 /label=polya misc_feature 2037..2077 /label=BBa_B0012 terminator 2037..2077 /label=BBa_B0012 misc_feature 2086..2105 /label=LuxR/HSL promoter 2105..2110 /label=-35 promoter 2127..2132 /label=-10 misc_feature 2138..2138 /label=start misc_feature 2086..2140 /label=BBa_R0062 promoter 2086..2140 /label=BBa_R0062 misc_feature 2153..2156 /label=conserved RBS 2149..2160 /label=BBa_B0034 CDS 2170..2786 /label=tetR misc_feature 2787..2820 /label=SsrA misc_feature 2827..2851 /label=Help:Barcodes CDS 2167..2826 /label=BBa_C0040 stem_loop 2871..2914 /label=stem_loop misc_feature 2860..2939 /label=BBa_B0010 terminator 2860..2939 /label=BBa_B0010 stem_loop 2955..2974 /label=T7 TE misc_feature 2981..2981 /label=stop polyA_site 2975..2988 /label=polya misc_feature 2948..2988 /label=BBa_B0012 terminator 2948..2988 /label=BBa_B0012 misc_feature 2997..3015 /label=TetR 1 misc_feature 2997..3050 /label=BBa_R0040 promoter 3016..3021 /label=-35 misc_feature 3022..3040 /label=TetR 2 promoter 3039..3044 /label=-10 promoter 2997..3050 /label=BBa_R0040 misc_feature 3063..3066 /label=conserved RBS 3059..3070 /label=BBa_B0034 misc_feature 3656..3687 /label=LVA CDS 3077..3655 /label=luxI misc_feature 3077..3694 /label=BBa_C0061 misc_feature 3695..3719 /label=Help:Barcodes CDS 3077..3694 /label=BBa_C0061 stem_loop 3739..3782 /label=stem_loop misc_feature 3728..3807 /label=BBa_B0010 terminator 3728..3807 /label=BBa_B0010 stem_loop 3823..3842 /label=T7 TE misc_feature 3849..3849 /label=stop polyA_site 3843..3856 /label=polya misc_feature 3816..3856 /label=BBa_B0012 terminator 3816..3856 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac 301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag 721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 781 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctgctgctgt ttaataatac 841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 961 tttctgcgtt tatatactag agacctgtac gatcctacag gtgcttatgt taagtaattg 1021 tattcccagc gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc 1081 attaccgttt taatgatata taacacgcaa aacttgcgac aaacaatagg taatactaga 1141 gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa 1201 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa 1261 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc 1321 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa 1381 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg 1441 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa 1501 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg 1561 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc 1621 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat 1681 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc 1741 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt 1801 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat 1861 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg 1921 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 1981 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2041 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg taggatcgta 2101 caggtttacg caagaaaatg gtttgttata gtcgaataaa tactagagaa agaggagaaa 2161 tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat 2221 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag 2281 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag 2341 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt 2401 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa 2461 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc 2521 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg 2581 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg 2641 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat 2701 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa 2761 aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct 2821 taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa 2881 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2941 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc 3001 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac tactagagaa 3061 agaggagaaa tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc 3121 ggaggagtat aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg 3181 ggacttagtt gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata 3241 tatttatgct tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac 3301 aggtgattat atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa 3361 agatcctaat atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa 3421 taactctgct agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag 3481 tcaaggtatt acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg 3541 tattaaagtt ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa 3601 atcggttgta ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaatgctgc 3661 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct 3721 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 3781 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 3841 cctttctgcg tttata //