LOCUS BBa_I13905 4325 bp DNA linear 22-Nov-2024 DEFINITION Ring Oscillator Synchronization Device (lac) ACCESSION BBa_I13905 VERSION BBa_I13905.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 832..837 /label=stop misc_feature 793..831 /label=AAV misc_feature 76..837 /label=BBa_E0034 stem_loop 857..900 /label=stem_loop misc_feature 846..925 /label=BBa_B0010 terminator 846..925 /label=BBa_B0010 stem_loop 941..960 /label=T7 TE misc_feature 967..967 /label=stop polyA_site 961..974 /label=polya misc_feature 934..974 /label=BBa_B0012 terminator 934..974 /label=BBa_B0012 misc_feature 983..1002 /label=LuxR/HSL promoter 1097..1104 /label=-10 promoter 1071..1076 /label=-35 misc_feature 1110..1110 /label=start misc_feature 1112..1131 /label=Putative LuxR/HSL misc_feature 983..1133 /label=BBa_R0063 promoter 983..1133 /label=BBa_R0063 misc_feature 1146..1149 /label=conserved RBS 1142..1153 /label=BBa_B0034 misc_feature 1160..1161 /label=prefix misc_feature 1333..1333 /label=T misc_feature 1651..1651 /label=A CDS 1160..1909 /label=luxR misc_feature 1160..1915 /label=BBa_C0062 misc_feature 1916..1940 /label=Help:Barcodes CDS 1160..1915 /label=BBa_C0062 stem_loop 1960..2003 /label=stem_loop misc_feature 1949..2028 /label=BBa_B0010 terminator 1949..2028 /label=BBa_B0010 stem_loop 2044..2063 /label=T7 TE misc_feature 2070..2070 /label=stop polyA_site 2064..2077 /label=polya misc_feature 2037..2077 /label=BBa_B0012 terminator 2037..2077 /label=BBa_B0012 misc_feature 2090..2093 /label=conserved RBS 2086..2097 /label=BBa_B0034 misc_feature 3193..3231 /label=LVA CDS 2104..3231 /label=lacI-LVA misc_feature 2104..3231 /label=BBa_C0012 misc_feature 3232..3256 /label=Help:Barcodes CDS 2104..3231 /label=BBa_C0012 stem_loop 3276..3319 /label=stem_loop misc_feature 3265..3344 /label=BBa_B0010 terminator 3265..3344 /label=BBa_B0010 stem_loop 3360..3379 /label=T7 TE misc_feature 3386..3386 /label=stop polyA_site 3380..3393 /label=polya misc_feature 3353..3393 /label=BBa_B0012 terminator 3353..3393 /label=BBa_B0012 misc_feature 3404..3420 /label=lac O1 promoter 3421..3426 /label=-35 misc_feature 3427..3443 /label=lac O1 promoter 3444..3449 /label=-10 misc_feature 3402..3455 /label=BBa_R0011 promoter 3402..3455 /label=BBa_R0011 misc_feature 3469..3472 /label=conserved RBS 3465..3476 /label=BBa_B0034 misc_feature 4062..4093 /label=LVA CDS 3483..4061 /label=luxI misc_feature 3483..4100 /label=BBa_C0061 misc_feature 4101..4125 /label=Help:Barcodes CDS 3483..4100 /label=BBa_C0061 stem_loop 4145..4188 /label=stem_loop misc_feature 4134..4213 /label=BBa_B0010 terminator 4134..4213 /label=BBa_B0010 stem_loop 4229..4248 /label=T7 TE misc_feature 4255..4255 /label=stop polyA_site 4249..4262 /label=polya misc_feature 4222..4262 /label=BBa_B0012 terminator 4222..4262 /label=BBa_B0012 misc_feature 4271..4290 /label=LuxR/HSL promoter 4290..4295 /label=-35 promoter 4312..4317 /label=-10 misc_feature 4323..4323 /label=start misc_feature 4271..4325 /label=BBa_R0062 promoter 4271..4325 /label=BBa_R0062 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac 301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag 721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 781 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctgctgctgt ttaataatac 841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 961 tttctgcgtt tatatactag agacctgtac gatcctacag gtgcttatgt taagtaattg 1021 tattcccagc gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc 1081 attaccgttt taatgatata taacacgcaa aacttgcgac aaacaatagg taatactaga 1141 gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa 1201 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa 1261 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc 1321 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa 1381 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg 1441 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa 1501 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg 1561 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc 1621 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat 1681 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc 1741 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt 1801 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat 1861 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg 1921 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 1981 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2041 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaaaga ggagaaatac 2101 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct 2161 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa 2221 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg 2281 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg 2341 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 2401 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg 2461 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 2521 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 2581 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 2641 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 2701 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 2761 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 2821 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 2881 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 2941 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg 3001 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 3061 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc 3121 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 3181 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 3241 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 3301 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 3361 gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag cggataacaa 3421 ttgacattgt gagcggataa caagatactg agcacatact agagaaagag gagaaatact 3481 agatgactat aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag 3541 gtattctaag tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag 3601 aaaataacct tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg 3661 atgatactga aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc 3721 tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag 3781 tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg 3841 aaattacaat gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag 3901 aatatgtaac agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt 3961 gtcatcgtat tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt 4021 ctatgcctat taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact 4081 acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca 4141 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 4201 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 4261 tatactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga 4321 ataaa //