LOCUS BBa_I13906 4331 bp DNA linear 22-Nov-2024 DEFINITION Synchronized Relaxation Oscillator ACCESSION BBa_I13906 VERSION BBa_I13906.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 838..843 /label=stop misc_feature 799..837 /label=AAV misc_feature 82..843 /label=BBa_E0034 stem_loop 863..906 /label=stem_loop misc_feature 852..931 /label=BBa_B0010 terminator 852..931 /label=BBa_B0010 stem_loop 947..966 /label=T7 TE misc_feature 973..973 /label=stop polyA_site 967..980 /label=polya misc_feature 940..980 /label=BBa_B0012 terminator 940..980 /label=BBa_B0012 misc_feature 989..1008 /label=LuxR/HSL promoter 1103..1110 /label=-10 promoter 1077..1082 /label=-35 misc_feature 1116..1116 /label=start misc_feature 1118..1137 /label=Putative LuxR/HSL misc_feature 989..1139 /label=BBa_R0063 promoter 989..1139 /label=BBa_R0063 misc_feature 1152..1155 /label=conserved RBS 1148..1159 /label=BBa_B0034 misc_feature 1166..1167 /label=prefix misc_feature 1339..1339 /label=T misc_feature 1657..1657 /label=A CDS 1166..1915 /label=luxR misc_feature 1166..1921 /label=BBa_C0062 misc_feature 1922..1946 /label=Help:Barcodes CDS 1166..1921 /label=BBa_C0062 stem_loop 1966..2009 /label=stem_loop misc_feature 1955..2034 /label=BBa_B0010 terminator 1955..2034 /label=BBa_B0010 stem_loop 2050..2069 /label=T7 TE misc_feature 2076..2076 /label=stop polyA_site 2070..2083 /label=polya misc_feature 2043..2083 /label=BBa_B0012 terminator 2043..2083 /label=BBa_B0012 misc_feature 2096..2099 /label=conserved RBS 2092..2103 /label=BBa_B0034 misc_feature 3199..3237 /label=LVA CDS 2110..3237 /label=lacI-LVA misc_feature 2110..3237 /label=BBa_C0012 misc_feature 3238..3262 /label=Help:Barcodes CDS 2110..3237 /label=BBa_C0012 stem_loop 3282..3325 /label=stem_loop misc_feature 3271..3350 /label=BBa_B0010 terminator 3271..3350 /label=BBa_B0010 stem_loop 3366..3385 /label=T7 TE misc_feature 3392..3392 /label=stop polyA_site 3386..3399 /label=polya misc_feature 3359..3399 /label=BBa_B0012 terminator 3359..3399 /label=BBa_B0012 misc_feature 3410..3426 /label=lac O1 promoter 3427..3432 /label=-35 misc_feature 3433..3449 /label=lac O1 promoter 3450..3455 /label=-10 misc_feature 3408..3461 /label=BBa_R0011 promoter 3408..3461 /label=BBa_R0011 misc_feature 3475..3478 /label=conserved RBS 3471..3482 /label=BBa_B0034 misc_feature 4068..4099 /label=LVA CDS 3489..4067 /label=luxI misc_feature 3489..4106 /label=BBa_C0061 misc_feature 4107..4131 /label=Help:Barcodes CDS 3489..4106 /label=BBa_C0061 stem_loop 4151..4194 /label=stem_loop misc_feature 4140..4219 /label=BBa_B0010 terminator 4140..4219 /label=BBa_B0010 stem_loop 4235..4254 /label=T7 TE misc_feature 4261..4261 /label=stop polyA_site 4255..4268 /label=polya misc_feature 4228..4268 /label=BBa_B0012 terminator 4228..4268 /label=BBa_B0012 misc_feature 4277..4296 /label=LuxR/HSL promoter 4296..4301 /label=-35 promoter 4318..4323 /label=-10 misc_feature 4329..4329 /label=start misc_feature 4277..4331 /label=BBa_R0062 promoter 4277..4331 /label=BBa_R0062 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgctgc tgctgtttaa 841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 961 tgggcctttc tgcgtttata tactagagac ctgtacgatc ctacaggtgc ttatgttaag 1021 taattgtatt cccagcgata caatagtgtg acaaaaatcc aatttattag aatcaaatgt 1081 caatccatta ccgttttaat gatatataac acgcaaaact tgcgacaaac aataggtaat 1141 actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat 1201 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat 1261 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt 1321 taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga 1381 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat 1441 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga 1501 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg 1561 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt 1621 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat 1681 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc 1741 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg 1801 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca 1861 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata 1921 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 1981 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2041 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaaagaggag 2101 aaatactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 2161 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 2221 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 2281 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 2341 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 2401 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 2461 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 2521 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 2581 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 2641 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 2701 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 2761 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 2821 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 2881 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 2941 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 3001 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 3061 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 3121 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 3181 cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 3241 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 3301 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3361 acactggctc accttcgggt gggcctttct gcgtttatat actagagaat tgtgagcgga 3421 taacaattga cattgtgagc ggataacaag atactgagca catactagag aaagaggaga 3481 aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca tcggaggagt 3541 ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag tgggacttag 3601 ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa tatatttatg 3661 cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca acaggtgatt 3721 atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc aaagatccta 3781 atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata aataactctg 3841 ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt agtcaaggta 3901 ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag cgtattaaag 3961 ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact aaatcggttg 4021 tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct gcaaacgacg 4081 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagagc 4141 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 4201 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 4261 cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat 4321 agtcgaataa a //