##gff-version 3
##sequence-region BBa_I13906 1 4331
BBa_I13906	.	CDS	1166	1921	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I13906	.	engineered_tag	1922	1946	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13906	.	engineered_region	1166	1167	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13906	.	point_mutation	1339	1339	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13906	.	engineered_region	1166	1921	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13906	.	point_mutation	1657	1657	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13906	.	CDS	1166	1915	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13906	.	ribosome_entry_site	1148	1159	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13906	.	conserved_region	1152	1155	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13906	.	engineered_region	82	843	.	+	0	ID=BBa_E0034;Name=BBa_E0034
BBa_I13906	.	tag	799	837	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I13906	.	stop_codon	838	843	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I13906	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13906	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13906	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I13906	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13906	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13906	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13906	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13906	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13906	.	terminator	3271	3350	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13906	.	stem_loop	3282	3325	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13906	.	engineered_region	3271	3350	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13906	.	terminator	3359	3399	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13906	.	stem_loop	3366	3385	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13906	.	polyA_site	3386	3399	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13906	.	stop_codon	3392	3392	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13906	.	engineered_region	3359	3399	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13906	.	promoter	4277	4331	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I13906	.	engineered_region	4277	4331	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13906	.	promoter	4296	4301	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13906	.	start_codon	4329	4329	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I13906	.	promoter	4318	4323	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I13906	.	non_covalent_binding_site	4277	4296	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I13906	.	ribosome_entry_site	2092	2103	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13906	.	conserved_region	2096	2099	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13906	.	promoter	3408	3461	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I13906	.	engineered_region	3408	3461	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13906	.	promoter	3450	3455	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13906	.	promoter	3427	3432	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13906	.	operator	3410	3426	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13906	.	operator	3433	3449	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13906	.	ribosome_entry_site	3471	3482	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13906	.	conserved_region	3475	3478	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13906	.	terminator	1955	2034	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13906	.	stem_loop	1966	2009	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13906	.	engineered_region	1955	2034	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13906	.	terminator	2043	2083	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13906	.	stem_loop	2050	2069	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13906	.	polyA_site	2070	2083	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13906	.	stop_codon	2076	2076	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13906	.	engineered_region	2043	2083	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13906	.	CDS	2110	3237	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I13906	.	engineered_tag	3238	3262	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13906	.	engineered_region	2110	3237	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13906	.	tag	3199	3237	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13906	.	CDS	2110	3237	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13906	.	terminator	852	931	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13906	.	stem_loop	863	906	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13906	.	engineered_region	852	931	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13906	.	terminator	940	980	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13906	.	stem_loop	947	966	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13906	.	polyA_site	967	980	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13906	.	stop_codon	973	973	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13906	.	engineered_region	940	980	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13906	.	CDS	3489	4106	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I13906	.	engineered_tag	4107	4131	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I13906	.	tag	4068	4099	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I13906	.	engineered_region	3489	4106	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I13906	.	CDS	3489	4067	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I13906	.	terminator	4140	4219	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13906	.	stem_loop	4151	4194	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13906	.	engineered_region	4140	4219	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13906	.	terminator	4228	4268	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13906	.	stem_loop	4235	4254	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13906	.	polyA_site	4255	4268	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13906	.	stop_codon	4261	4261	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13906	.	engineered_region	4228	4268	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13906	.	promoter	989	1139	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I13906	.	promoter	1103	1110	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I13906	.	non_covalent_binding_site	1118	1137	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I13906	.	non_covalent_binding_site	989	1008	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I13906	.	start_codon	1116	1116	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I13906	.	engineered_region	989	1139	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I13906	.	promoter	1077	1082	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
>BBa_I13906
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggcca
caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggc
aagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccaca
tgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg
caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttc
aaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagc
agaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacca
gcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaa
gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagc
tgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgt
attcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatata
taacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccga
cgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgact
aaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctag
ataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaa
ctccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaa
gcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttg
cacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctct
agttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgttta
gcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatt
taaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattga
ttgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacg
ttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctg
cgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcggg
caaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaa
tctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcgg
tgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtgga
agctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccat
gaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaa
gttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg
ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatg
ctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatct
cggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcct
gctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtc
tcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc
agctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataact
ctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagag
gagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaa
gtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatga
taactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaaca
ggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaat
taagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagc
tatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaag
cgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtcta
tgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataact
ctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa