LOCUS BBa_I13907 4325 bp DNA linear 22-Nov-2024 DEFINITION Ring Oscillator Synchronization Device (lac) ACCESSION BBa_I13907 VERSION BBa_I13907.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 832..837 /label=stop misc_feature 793..831 /label=AAV misc_feature 76..837 /label=BBa_E0034 stem_loop 857..900 /label=stem_loop misc_feature 846..925 /label=BBa_B0010 terminator 846..925 /label=BBa_B0010 stem_loop 941..960 /label=T7 TE misc_feature 967..967 /label=stop polyA_site 961..974 /label=polya misc_feature 934..974 /label=BBa_B0012 terminator 934..974 /label=BBa_B0012 misc_feature 983..1002 /label=LuxR/HSL promoter 1097..1104 /label=-10 promoter 1071..1076 /label=-35 misc_feature 1110..1110 /label=start misc_feature 1112..1131 /label=Putative LuxR/HSL misc_feature 983..1133 /label=BBa_R0063 promoter 983..1133 /label=BBa_R0063 misc_feature 1146..1149 /label=conserved RBS 1142..1153 /label=BBa_B0034 misc_feature 1160..1161 /label=prefix misc_feature 1333..1333 /label=T misc_feature 1651..1651 /label=A CDS 1160..1909 /label=luxR misc_feature 1160..1915 /label=BBa_C0062 misc_feature 1916..1940 /label=Help:Barcodes CDS 1160..1915 /label=BBa_C0062 stem_loop 1960..2003 /label=stem_loop misc_feature 1949..2028 /label=BBa_B0010 terminator 1949..2028 /label=BBa_B0010 stem_loop 2044..2063 /label=T7 TE misc_feature 2070..2070 /label=stop polyA_site 2064..2077 /label=polya misc_feature 2037..2077 /label=BBa_B0012 terminator 2037..2077 /label=BBa_B0012 misc_feature 2086..2105 /label=LuxR/HSL promoter 2105..2110 /label=-35 promoter 2127..2132 /label=-10 misc_feature 2138..2138 /label=start misc_feature 2086..2140 /label=BBa_R0062 promoter 2086..2140 /label=BBa_R0062 misc_feature 2153..2156 /label=conserved RBS 2149..2160 /label=BBa_B0034 misc_feature 3256..3294 /label=LVA CDS 2167..3294 /label=lacI-LVA misc_feature 2167..3294 /label=BBa_C0012 misc_feature 3295..3319 /label=Help:Barcodes CDS 2167..3294 /label=BBa_C0012 stem_loop 3339..3382 /label=stem_loop misc_feature 3328..3407 /label=BBa_B0010 terminator 3328..3407 /label=BBa_B0010 stem_loop 3423..3442 /label=T7 TE misc_feature 3449..3449 /label=stop polyA_site 3443..3456 /label=polya misc_feature 3416..3456 /label=BBa_B0012 terminator 3416..3456 /label=BBa_B0012 misc_feature 3467..3483 /label=lac O1 promoter 3484..3489 /label=-35 misc_feature 3490..3506 /label=lac O1 promoter 3507..3512 /label=-10 misc_feature 3465..3518 /label=BBa_R0011 promoter 3465..3518 /label=BBa_R0011 misc_feature 3532..3535 /label=conserved RBS 3528..3539 /label=BBa_B0034 misc_feature 4125..4156 /label=LVA CDS 3546..4124 /label=luxI misc_feature 3546..4163 /label=BBa_C0061 misc_feature 4164..4188 /label=Help:Barcodes CDS 3546..4163 /label=BBa_C0061 stem_loop 4208..4251 /label=stem_loop misc_feature 4197..4276 /label=BBa_B0010 terminator 4197..4276 /label=BBa_B0010 stem_loop 4292..4311 /label=T7 TE misc_feature 4318..4318 /label=stop polyA_site 4312..4325 /label=polya misc_feature 4285..4325 /label=BBa_B0012 terminator 4285..4325 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac 301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag 721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 781 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctgctgctgt ttaataatac 841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 961 tttctgcgtt tatatactag agacctgtac gatcctacag gtgcttatgt taagtaattg 1021 tattcccagc gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc 1081 attaccgttt taatgatata taacacgcaa aacttgcgac aaacaatagg taatactaga 1141 gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa 1201 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa 1261 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc 1321 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa 1381 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg 1441 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa 1501 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg 1561 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc 1621 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat 1681 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc 1741 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt 1801 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat 1861 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg 1921 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 1981 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2041 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg taggatcgta 2101 caggtttacg caagaaaatg gtttgttata gtcgaataaa tactagagaa agaggagaaa 2161 tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc 2221 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg 2281 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa 2341 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg 2401 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg 2461 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc 2521 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct 2581 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc 2641 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc 2701 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt 2761 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa 2821 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag 2881 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc 2941 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat 3001 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg 3061 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc 3121 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa 3181 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 3241 ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga 3301 tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 3361 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3421 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagaattgt gagcggataa 3481 caattgacat tgtgagcgga taacaagata ctgagcacat actagagaaa gaggagaaat 3541 actagatgac tataatgata aaaaaatcgg attttttggc aattccatcg gaggagtata 3601 aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg gacttagttg 3661 tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat atttatgctt 3721 gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca ggtgattata 3781 tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa gatcctaata 3841 tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc aaagataaat aactctgcta 3901 gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt caaggtatta 3961 cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt attaaagttc 4021 cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa tcggttgtat 4081 tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaatgctgca aacgacgaaa 4141 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag 4201 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 4261 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4321 ttata //