LOCUS BBa_I13908 4331 bp DNA linear 17-May-2024 DEFINITION Synchronized Relaxation Oscillator ACCESSION BBa_I13908 VERSION BBa_I13908.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 838..843 /label=stop misc_feature 799..837 /label=AAV misc_feature 82..843 /label=BBa_E0034 stem_loop 863..906 /label=stem_loop misc_feature 852..931 /label=BBa_B0010 terminator 852..931 /label=BBa_B0010 stem_loop 947..966 /label=T7 TE misc_feature 973..973 /label=stop polyA_site 967..980 /label=polya misc_feature 940..980 /label=BBa_B0012 terminator 940..980 /label=BBa_B0012 misc_feature 989..1008 /label=LuxR/HSL promoter 1103..1110 /label=-10 promoter 1077..1082 /label=-35 misc_feature 1116..1116 /label=start misc_feature 1118..1137 /label=Putative LuxR/HSL misc_feature 989..1139 /label=BBa_R0063 promoter 989..1139 /label=BBa_R0063 misc_feature 1152..1155 /label=conserved RBS 1148..1159 /label=BBa_B0034 misc_feature 1166..1167 /label=prefix misc_feature 1339..1339 /label=T misc_feature 1657..1657 /label=A CDS 1166..1915 /label=luxR misc_feature 1166..1921 /label=BBa_C0062 misc_feature 1922..1946 /label=Help:Barcodes CDS 1166..1921 /label=BBa_C0062 stem_loop 1966..2009 /label=stem_loop misc_feature 1955..2034 /label=BBa_B0010 terminator 1955..2034 /label=BBa_B0010 stem_loop 2050..2069 /label=T7 TE misc_feature 2076..2076 /label=stop polyA_site 2070..2083 /label=polya misc_feature 2043..2083 /label=BBa_B0012 terminator 2043..2083 /label=BBa_B0012 misc_feature 2092..2111 /label=LuxR/HSL promoter 2111..2116 /label=-35 promoter 2133..2138 /label=-10 misc_feature 2144..2144 /label=start misc_feature 2092..2146 /label=BBa_R0062 promoter 2092..2146 /label=BBa_R0062 misc_feature 2159..2162 /label=conserved RBS 2155..2166 /label=BBa_B0034 misc_feature 3262..3300 /label=LVA CDS 2173..3300 /label=lacI-LVA misc_feature 2173..3300 /label=BBa_C0012 misc_feature 3301..3325 /label=Help:Barcodes CDS 2173..3300 /label=BBa_C0012 stem_loop 3345..3388 /label=stem_loop misc_feature 3334..3413 /label=BBa_B0010 terminator 3334..3413 /label=BBa_B0010 stem_loop 3429..3448 /label=T7 TE misc_feature 3455..3455 /label=stop polyA_site 3449..3462 /label=polya misc_feature 3422..3462 /label=BBa_B0012 terminator 3422..3462 /label=BBa_B0012 misc_feature 3473..3489 /label=lac O1 promoter 3490..3495 /label=-35 misc_feature 3496..3512 /label=lac O1 promoter 3513..3518 /label=-10 misc_feature 3471..3524 /label=BBa_R0011 promoter 3471..3524 /label=BBa_R0011 misc_feature 3538..3541 /label=conserved RBS 3534..3545 /label=BBa_B0034 misc_feature 4131..4162 /label=LVA CDS 3552..4130 /label=luxI misc_feature 3552..4169 /label=BBa_C0061 misc_feature 4170..4194 /label=Help:Barcodes CDS 3552..4169 /label=BBa_C0061 stem_loop 4214..4257 /label=stem_loop misc_feature 4203..4282 /label=BBa_B0010 terminator 4203..4282 /label=BBa_B0010 stem_loop 4298..4317 /label=T7 TE misc_feature 4324..4324 /label=stop polyA_site 4318..4331 /label=polya misc_feature 4291..4331 /label=BBa_B0012 terminator 4291..4331 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgctgc tgctgtttaa 841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 961 tgggcctttc tgcgtttata tactagagac ctgtacgatc ctacaggtgc ttatgttaag 1021 taattgtatt cccagcgata caatagtgtg acaaaaatcc aatttattag aatcaaatgt 1081 caatccatta ccgttttaat gatatataac acgcaaaact tgcgacaaac aataggtaat 1141 actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat 1201 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat 1261 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt 1321 taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga 1381 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat 1441 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga 1501 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg 1561 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt 1621 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat 1681 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc 1741 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg 1801 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca 1861 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata 1921 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 1981 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2041 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacctgtagg 2101 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaatact agagaaagag 2161 gagaaatact agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc 2221 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 2281 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 2341 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 2401 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 2461 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 2521 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 2581 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 2641 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 2701 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 2761 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 2821 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 2881 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 2941 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 3001 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 3061 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 3121 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 3181 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 3241 tcccgactgg aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa 3301 ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc 3361 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3421 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattgtgagc 3481 ggataacaat tgacattgtg agcggataac aagatactga gcacatacta gagaaagagg 3541 agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg 3601 agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact 3661 tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt 3721 atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg 3781 attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc 3841 ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact 3901 ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag 3961 gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta 4021 aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg 4081 ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg 4141 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 4201 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 4261 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 4321 ctgcgtttat a //