LOCUS BBa_I13920 3343 bp DNA linear 24-Nov-2024 DEFINITION Ring Oscillator Synchronization Device (I13260.Q04121.I0461) ACCESSION BBa_I13920 VERSION BBa_I13920.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 2274..2312 /label=LVA CDS 1185..2312 /label=lacI-LVA misc_feature 1185..2312 /label=BBa_C0012 misc_feature 2313..2337 /label=Help:Barcodes CDS 1185..2312 /label=BBa_C0012 stem_loop 2357..2400 /label=stem_loop misc_feature 2346..2425 /label=BBa_B0010 terminator 2346..2425 /label=BBa_B0010 stem_loop 2441..2460 /label=T7 TE misc_feature 2467..2467 /label=stop polyA_site 2461..2474 /label=polya misc_feature 2434..2474 /label=BBa_B0012 terminator 2434..2474 /label=BBa_B0012 terminator 2346..2474 /label=BBa_B0015 misc_feature 2485..2501 /label=lac O1 promoter 2502..2507 /label=-35 misc_feature 2508..2524 /label=lac O1 promoter 2525..2530 /label=-10 misc_feature 2483..2536 /label=BBa_R0011 promoter 2483..2536 /label=BBa_R0011 misc_feature 1167..2537 /label=BBa_Q04121 misc_feature 2550..2553 /label=conserved RBS 2546..2557 /label=BBa_B0034 misc_feature 3143..3174 /label=LVA CDS 2564..3142 /label=luxI misc_feature 2564..3181 /label=BBa_C0061 misc_feature 3182..3206 /label=Help:Barcodes CDS 2564..3181 /label=BBa_C0061 stem_loop 3226..3269 /label=stem_loop misc_feature 3215..3294 /label=BBa_B0010 terminator 3215..3294 /label=BBa_B0010 stem_loop 3310..3329 /label=T7 TE misc_feature 3336..3336 /label=stop polyA_site 3330..3343 /label=polya misc_feature 3303..3343 /label=BBa_B0012 terminator 3303..3343 /label=BBa_B0012 misc_feature 2546..3343 /label=BBa_F1610 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatggtg aatgtgaaac 1201 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 1261 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 1321 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 1381 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 1441 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 1501 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 1561 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 1621 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 1681 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 1741 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 1801 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 1861 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 1921 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 1981 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 2041 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 2101 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 2161 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 2221 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa 2281 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac 2341 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2401 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2461 tttctgcgtt tatatactag agaattgtga gcggataaca attgacattg tgagcggata 2521 acaagatact gagcacatac tagagaaaga ggagaaatac tagatgacta taatgataaa 2581 aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta 2641 tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga 2701 tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag 2761 tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga 2821 attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc 2881 tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt 2941 tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc 3001 aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa 3061 agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca 3121 gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata 3181 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct 3241 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 3301 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //