LOCUS BBa_I13921 2874 bp DNA linear 25-Nov-2024 DEFINITION Ring Oscillator Synchronization Device (I13260.Q04400.I0461) ACCESSION BBa_I13921 VERSION BBa_I13921.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 CDS 1188..1804 /label=tetR misc_feature 1805..1838 /label=SsrA misc_feature 1845..1869 /label=Help:Barcodes CDS 1185..1844 /label=BBa_C0040 stem_loop 1889..1932 /label=stem_loop misc_feature 1878..1957 /label=BBa_B0010 terminator 1878..1957 /label=BBa_B0010 stem_loop 1973..1992 /label=T7 TE misc_feature 1999..1999 /label=stop polyA_site 1993..2006 /label=polya misc_feature 1966..2006 /label=BBa_B0012 terminator 1966..2006 /label=BBa_B0012 misc_feature 2015..2033 /label=TetR 1 misc_feature 2015..2068 /label=BBa_R0040 promoter 2034..2039 /label=-35 misc_feature 2040..2058 /label=TetR 2 promoter 2057..2062 /label=-10 promoter 2015..2068 /label=BBa_R0040 misc_feature 2081..2084 /label=conserved RBS 2077..2088 /label=BBa_B0034 misc_feature 2674..2705 /label=LVA CDS 2095..2673 /label=luxI misc_feature 2095..2712 /label=BBa_C0061 misc_feature 2713..2737 /label=Help:Barcodes CDS 2095..2712 /label=BBa_C0061 stem_loop 2757..2800 /label=stem_loop misc_feature 2746..2825 /label=BBa_B0010 terminator 2746..2825 /label=BBa_B0010 stem_loop 2841..2860 /label=T7 TE misc_feature 2867..2867 /label=stop polyA_site 2861..2874 /label=polya misc_feature 2834..2874 /label=BBa_B0012 terminator 2834..2874 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgtcc agattagata 1201 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 1261 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 1321 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 1381 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 1441 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 1501 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 1561 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 1621 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 1681 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 1741 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 1801 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt 1861 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1921 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1981 ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat 2041 ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata ctagatgact 2101 ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta 2161 agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac 2221 cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact 2281 gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt 2341 gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta 2401 agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca 2461 atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta 2521 acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt 2581 attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct 2641 attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta 2701 gtagcttaat aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa 2761 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2821 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //