LOCUS BBa_I13922 2959 bp DNA linear 22-Dec-2024 DEFINITION Ring Oscillator Synchronization Device (I13260.Q04510.I0461) ACCESSION BBa_I13922 VERSION BBa_I13922.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 CDS 1188..1895 /label=cI lambda misc_feature 1896..1928 /label=LVA misc_feature 1935..1959 /label=Help:Barcodes CDS 1185..1934 /label=BBa_C0051 stem_loop 1979..2022 /label=stem_loop misc_feature 1968..2047 /label=BBa_B0010 terminator 1968..2047 /label=BBa_B0010 stem_loop 2063..2082 /label=T7 TE misc_feature 2089..2089 /label=stop polyA_site 2083..2096 /label=polya misc_feature 2056..2096 /label=BBa_B0012 terminator 2056..2096 /label=BBa_B0012 promoter 2142..2147 /label=-10 promoter 2119..2124 /label=-35 misc_feature 2129..2145 /label=OR1 misc_feature 2105..2121 /label=OR2 misc_feature 2105..2153 /label=BBa_R0051 promoter 2105..2153 /label=BBa_R0051 misc_feature 2166..2169 /label=conserved RBS 2162..2173 /label=BBa_B0034 misc_feature 2759..2790 /label=LVA CDS 2180..2758 /label=luxI misc_feature 2180..2797 /label=BBa_C0061 misc_feature 2798..2822 /label=Help:Barcodes CDS 2180..2797 /label=BBa_C0061 stem_loop 2842..2885 /label=stem_loop misc_feature 2831..2910 /label=BBa_B0010 terminator 2831..2910 /label=BBa_B0010 stem_loop 2926..2945 /label=T7 TE misc_feature 2952..2952 /label=stop polyA_site 2946..2959 /label=polya misc_feature 2919..2959 /label=BBa_B0012 terminator 2919..2959 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgagc acaaaaaaga 1201 aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa 1261 agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt 1321 caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc 1381 ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct 1441 acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg 1501 ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg 1561 atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg 1621 ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt 1681 taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg 1741 ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac 1801 cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag 1861 ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt 1921 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat 1981 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2041 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2101 agagtaacac cgtgcgtgtt gactatttta cctctggcgg tgataatggt tgctactaga 2161 gaaagaggag aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc 2221 atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga 2281 gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga 2341 atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac 2401 aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc 2461 caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat 2521 aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt 2581 tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa 2641 gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac 2701 taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc 2761 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc 2821 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2881 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2941 gggcctttct gcgtttata //