LOCUS       BBa_I13941              1820 bp    DNA     linear       22-Nov-2024
DEFINITION  Exogenous LuxI Relaxation Oscillator Test Construct Intermediate 
            (R0062.C0260.E0424)
ACCESSION   BBa_I13941
VERSION     BBa_I13941.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=start
     misc_feature    865..870
                     /label=2
     misc_feature    832..864
                     /label=LVA
     CDS             82..831
                     /label=aiiA
     misc_feature    82..870
                     /label=BBa_C0060
     misc_feature    871..895
                     /label=Help:Barcodes
     CDS             82..870
                     /label=BBa_C0060
     misc_feature    908..911
                     /label=conserved
     RBS             904..915
                     /label=BBa_B0034
     misc_feature    922..1683
                     /label=BBa_E0024
     stem_loop       1703..1746
                     /label=stem_loop
     misc_feature    1692..1771
                     /label=BBa_B0010
     terminator      1692..1771
                     /label=BBa_B0010
     stem_loop       1787..1806
                     /label=T7 TE
     misc_feature    1813..1813
                     /label=stop
     polyA_site      1807..1820
                     /label=polya
     misc_feature    1780..1820
                     /label=BBa_B0012
     terminator      1780..1820
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt
      121 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta
      181 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg
      241 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt
      301 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag
      361 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat
      421 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat
      481 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt
      541 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat
      601 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg
      661 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct
      721 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt
      781 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac
      841 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      901 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
      961 cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag
     1021 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1081 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc
     1141 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1201 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1261 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1321 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc
     1381 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     1441 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1501 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     1561 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1621 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgctgc tgctgtttaa
     1681 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1741 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1801 tgggcctttc tgcgtttata
//