LOCUS       BBa_I13942              2975 bp    DNA     linear       10-May-2024
DEFINITION  Exogenous AiiA Relaxation Oscillator Test Construct Intermediate 
            (I0500.C0260.E0424)
ACCESSION   BBa_I13942
VERSION     BBa_I13942.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     misc_feature    1237..1239
                     /label=start
     misc_feature    2020..2025
                     /label=2
     misc_feature    1987..2019
                     /label=LVA
     CDS             1237..1986
                     /label=aiiA
     misc_feature    1237..2025
                     /label=BBa_C0060
     misc_feature    2026..2050
                     /label=Help:Barcodes
     CDS             1237..2025
                     /label=BBa_C0060
     misc_feature    2063..2066
                     /label=conserved
     RBS             2059..2070
                     /label=BBa_B0034
     misc_feature    2077..2838
                     /label=BBa_E0024
     stem_loop       2858..2901
                     /label=stem_loop
     misc_feature    2847..2926
                     /label=BBa_B0010
     terminator      2847..2926
                     /label=BBa_B0010
     stem_loop       2942..2961
                     /label=T7 TE
     misc_feature    2968..2968
                     /label=stop
     polyA_site      2962..2975
                     /label=polya
     misc_feature    2935..2975
                     /label=BBa_B0012
     terminator      2935..2975
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga cagtaaagaa gctttatttc
     1261 gtcccagcag gtcgttgtat gttggatcat tcgtctgtta atagtacatt aacaccagga
     1321 gaattattag acttaccggt ttggtgttat cttttggaga ctgaagaagg acctatttta
     1381 gtagatacag gtatgccaga aagtgcagtt aataatgaag gtctttttaa cggtacattt
     1441 gtcgaagggc aggttttacc gaaaatgact gaagaagata gaatcgtgaa tattttaaaa
     1501 cgggttggtt atgagccgga agaccttctt tatattatta gttctcactt gcattttgat
     1561 catgcaggag gaaatggcgc ttttataaat acaccaatca ttgtacagcg tgctgaatat
     1621 gaggcggcgc agcatagcga agaatatttg aaagaatgta tattgccgaa tttaaactac
     1681 aaaatcattg aaggtgatta tgaagtcgta ccaggagttc aattattgca tacaccaggc
     1741 catactccag ggcatcaatc gctattaatt gagacagaaa aatccggtcc tgtattatta
     1801 acgattgatg catcgtatac gaaagagaat tttgaaaatg aagtgccatt tgcgggattt
     1861 gattcagaat tagctttatc ttcaattaaa cgtttaaaag aagtggtgat gaaagagaag
     1921 ccgattgttt tctttggaca tgatatagag caggaaaggg gatgtaaagt gttccctgaa
     1981 tatatagctg caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag
     2041 tgtagatcgc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg
     2101 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc
     2161 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     2221 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
     2281 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2341 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2401 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2461 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
     2521 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     2581 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2641 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2701 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2761 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac
     2821 gctgctgctg tttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2881 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2941 tggctcacct tcgggtgggc ctttctgcgt ttata
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