LOCUS       BBa_I13943              1649 bp    DNA     linear       22-Nov-2024
DEFINITION  Exogenous AiiA Relaxation Oscillator Test Construct Intermediate 
            (R0062.B0034.C0061.E0434)
ACCESSION   BBa_I13943
VERSION     BBa_I13943.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    661..692
                     /label=LVA
     CDS             82..660
                     /label=luxI
     misc_feature    82..699
                     /label=BBa_C0061
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             82..699
                     /label=BBa_C0061
     misc_feature    737..740
                     /label=conserved
     RBS             733..744
                     /label=BBa_B0034
     misc_feature    1507..1512
                     /label=stop
     misc_feature    1468..1506
                     /label=AAV
     misc_feature    751..1512
                     /label=BBa_E0034
     stem_loop       1532..1575
                     /label=stem_loop
     misc_feature    1521..1600
                     /label=BBa_B0010
     terminator      1521..1600
                     /label=BBa_B0010
     stem_loop       1616..1635
                     /label=T7 TE
     misc_feature    1642..1642
                     /label=stop
     polyA_site      1636..1649
                     /label=polya
     misc_feature    1609..1649
                     /label=BBa_B0012
     terminator      1609..1649
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      721 tctctactag agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc
      781 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      841 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      901 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa
      961 tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc
     1021 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1081 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1141 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1201 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1261 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1321 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     1381 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1441 actctcggca tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgctgct
     1501 gctgtttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1561 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1621 accttcgggt gggcctttct gcgtttata
//